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  • MAB3326 - Anti-Collagen Type IV Antibody, 7S Domain, clone IV-4H12

MAB3326 Sigma-Aldrich

Anti-Collagen Type IV Antibody, 7S Domain, clone IV-4H12

clone IV-4H12, Chemicon®, from mouse

  •  eCl@ss 32160702

  •  NACRES NA.41



Related Categories Alphabetical Index, Antibodies, CO-CP, Primary Antibodies
clone   IV-4H12, monoclonal
biological source   mouse
application(s)   ELISA: suitable
  immunohistochemistry: suitable (paraffin)
  western blot: suitable
species reactivity   human
should not react with   rat
shipped in   dry ice
isotype   IgG1κ
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_001845.4
UniProt accession no.   P02462
Gene Information   human ... COL4A1(1282)



Specifically reactive with human Type IV Collagen.


Epitope: 7S Domain


Research Category
Cell Structure

Research Sub Category
ECM Proteins

This Anti-Collagen Type IV Antibody, 7S Domain, clone IV-4H12 is validated for use in ELISA, WB, IH(P) for the detection of Collagen Type IV.

Western blot. Detects human type IV collagen from human placental tissues at 2 μg/mL, giving a band at approximately 92 kDa. Optimal working dilutions must be determined by end user.

Physical form

Format: Purified

Liquid in 0.1 M sodium phosphate buffer, pH 7.0, containing 2% protease-free bovine serum albumin.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 12 months.


Manufactured by Daiichi Fine Chemical Co., Ltd

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Analysis Note

POSITIVE CONTROL: human placental tissues.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


Antibody Basics

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Western Blot Protocol | Immunoblotting Protocol

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Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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