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  • MAB3326 - Anti-Collagen Type IV Antibody, 7S Domain, clone IV-4H12

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MAB3326 Sigma-Aldrich

Anti-Collagen Type IV Antibody, 7S Domain, clone IV-4H12

clone IV-4H12, Chemicon®, from mouse

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, CO-CP, Primary Antibodies
clone   IV-4H12, monoclonal
biological source   mouse
application(s)   ELISA: suitable
  immunohistochemistry: suitable (paraffin)
  western blot: suitable
species reactivity   human
should not react with   rat
shipped in   dry ice
isotype   IgG1κ
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_001845.4
UniProt accession no.   P02462
Gene Information   human ... COL4A1(1282)

Description

Specificity

Specifically reactive with human Type IV Collagen.

Immunogen

Epitope: 7S Domain

Application

Research Category
Cell Structure

Research Sub Category
ECM Proteins

This Anti-Collagen Type IV Antibody, 7S Domain, clone IV-4H12 is validated for use in ELISA, WB, IH(P) for the detection of Collagen Type IV.

Western blot. Detects human type IV collagen from human placental tissues at 2 μg/mL, giving a band at approximately 92 kDa. Optimal working dilutions must be determined by end user.

Physical form

Format: Purified

Liquid in 0.1 M sodium phosphate buffer, pH 7.0, containing 2% protease-free bovine serum albumin.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 12 months.

Disclaimer

Manufactured by Daiichi Fine Chemical Co., Ltd

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Analysis Note

Control
POSITIVE CONTROL: human placental tissues.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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