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  • MAB3369 - Anti-MMP-3 Antibody, clone SL-1 IID4

MAB3369 Sigma-Aldrich

Anti-MMP-3 Antibody, clone SL-1 IID4

clone SL-1 IID4, Chemicon®, from mouse

Synonym: Stromelysin-1, Transin, Proteoglycanase

  •  eCl@ss 32160702



Related Categories Alphabetical Index, Antibodies, MF-MN, Primary Antibodies
clone   SL-1 IID4, monoclonal
biological source   mouse
application(s)   ELISA: suitable
  immunohistochemistry: suitable (paraffin)
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   human
shipped in   wet ice
isotype   IgG2b
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_002422.3
UniProt accession no.   P08254
Gene Information   human ... MMP3(4314)


General description

MMPs are frequently expressed in malignant neoplastic cells and play crucial roles in tumor invasion and metastasis. Tissue inhibitors of matrix metalloproteinases (TIMPs) control the activitiy of MMPS. The tissue distribution of three major MMPs has been defined in human mammary pathology: 1) collagenase (MMP-1) which degrades fibrillar interstitial collagens; 2) a 72-k/Da gelatinase (MMP-2) which mainly degrades type IV collagen and denatured collagens; and 3) stromelysin (MMP-3) which has a wider range of action, degrading several matrix components including the core proteins of proteoglycans, laminin and non-helical regions of collagens.


MAB3369 recognizes one doublet of 54kDa/59kDa and another doublet of 44kDa/49kDa, which are identified as unglycosylated and glycosylated species of pro (latent) and active forms of matrix metalloproteinase-3 (MMP-3; also known as Stromelysin-1 of Transin). MAB3369 shows no cross-reaction with the pro and active forms of other MMPs.




APMA (4-Aminophenylmercuric acetate) activated Human stromelysin-1


Anti-MMP-3 Antibody, clone SL-1 IID4 is an antibody against MMP-3 for use in ELISA, IP, WB, IH(P).


Immunoprecipitation (Use Protein A): 2μg/ml protein lysate.

Western blotting: 0.5-1.0 μg/ml for 2hrs at RT

Immunohistology (frozen & formalin-fixed): 10-20 μg/ml for 60 minute at room temperature. No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissue sections.

Optimal working dilutions and protocols must be determined by end user.

Research Category
Cell Structure

Research Sub Category

Physical form

Format: Purified

Purified from ascites fluid by Protein A chromatography. 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Storage and Stability

Antibody with sodium azide is stable for 12 months when stored at 2-8°C.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Analysis Note


Human endometrium cells, placenta, bladder, breast, ovarian carcinomas.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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