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  • MAB342 - Anti-Galactocerebroside Antibody, clone mGalC

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MAB342 Sigma-Aldrich

Anti-Galactocerebroside Antibody, clone mGalC

Synonym: Galactoceramide, Galactocerebroside, Galactosylceramide, GalC

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Antibodies, Antibodies for Neurobiology, Antibodies to Neural Proteins, Miscellaneous, Neuroscience,
Quality Level   100
biological source   mouse
clone   mGalC, monoclonal
species reactivity   chicken, rabbit, mouse, bovine, human, rat
mfr. no.   Chemicon®
application(s)   ELISA: suitable
  immunocytochemistry: suitable
  immunohistochemistry: suitable
isotype   IgG3
shipped in   dry ice

Description

General description

Galactocerebroside (GalC) is a major galactosphingolipid of myelin which plays a role in myelination. GalC is a very useful, specific marker for oligodendroglial lineage.

Specificity

Galactocerebroside (GalC), sulfatide, psychosine and other galactolipids. Cross-reacts with the sulfatide ester of GalC, but to a 16-fold lesser extent. No cross-reactivity with sphingosine, ceramide, mixed ganglioside or glucocerebroside. Binds specifically with oligodendrocytes and Schwann cells.

Immunogen

Synaptic plasma membranes from bovine hippocampus. Ranscht, B. et al. PNAS 79(8):2709-2713 (1982)

Application

Immunohistochemistry:
0.5-10 μg/mL of a previous lot was used on formalin fixed frozen sections of a prevous lot. Can not be used on paraffin embedded tissue sections since the antigen is denatured during embedding and paraffin removal.

Immunocytochemistry:
0.5-10 μg/mL of a previous lot was used on 4% paraformaldehyde, acetic acid or ethanol fixed cultured cells.

ELISA:
A previous lot was used on purified galactocerebrosides.

Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Neuronal & Glial Markers

Physical form

Format: Purified

Protein A Purfied

Purified mouse monoclonal in buffer containing 10 mM Potassium Phosphate, 150 mM NaCl, pH 7.4 containing 0.09% sodium azide.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
Neonatal cortex

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Derivation of Functional Oligodendrocyte Progenitor Cells (OPCs) from Human Neural Stem Cell Lines

Introduction Methods    Human Neural Stem Cell Culture    Oligodendrocyte Differentiation and Maturation    Oligodendrocyte Progenitor Cell Characterization    In Vitro Myelination Assay Results Conc...
Christine Chen, Michael Moeller, Anna Abai, Nick Asbrock and Vi Chu1
1
MilliporeSigma, Bioscience Division, Temecula, CA, USA
 
Keywords: Adhesion, Atomic absorption spectroscopy, Cell culture, Cell proliferation, Central Nervous System, Clinical, Culture media, Diseases, Gene expression, Growth factors, Hormones, Neurodegenerative Diseases, Pharmaceutical, Phase transitions, RNA immunoprecipitation, Transfection

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Peer-Reviewed Papers
15

References

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