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  • MAB347 - Anti-Growth Associated Protein 43 Antibody, clone 9-1E12

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MAB347 Sigma-Aldrich

Anti-Growth Associated Protein 43 Antibody, clone 9-1E12

Synonym: Neuromodulin, B-50 Protein, Growth Associated Protein 43, neuromodulin

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, GQ-GZ, Primary Antibodies
clone   9-1E12, monoclonal
biological source   mouse
application(s)   immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   hamster, human, hamster, rat, feline, rat, feline
species reactivity (predicted by homology)   human
shipped in   wet ice
isotype   IgG1
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_002045.2
UniProt accession no.   P17677
Gene Information   human ... GAP43(2596)

Description

General description

GAP-43, a common marker of differentiating neurons, is expressed at elevated levels by developing or regenerating neurons during axon growth. While GAP-43 is an integral membrane protein associated with the cytoplasmic surface of axonal growth cones (and synapses), it is absent from dendritic growth cones. In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. Phosphorylation of GAP-43 by PKC (at Ser41 on human, mouse, rat, and bovine GAP-43 or Ser42 on chicken and Xenopus GAP-43) is specifically correlated with certain forms of synaptic plasticity.

Specificity

GAP-43 (also known as growth associated protein-43, B-50, F1 and pp46), regardless of the protein’s phosphorylation state.

Immunogen

GAP-43 purified from rat brain

Application

Immunohistochemistry:
0.1-0.5 µg/mL of a previous lot on 4% paraformaldehyde perfused and fixed rat cerebellum and cerebrum tissue. The addition of 0.01-0.1% Triton X-100 to incubation buffer will increase cellular permeability.

Immunoprecipitation:
A previous lot of this antibody was used in immunoprecipitation.
5-10µg/mL in membrane preparations from 16-18 day embryonic cortical neuronal cultures.

Western Blot Analysis:
MAB347 will detect a single 43-48 kD band in western blots of membrane fractions of growing neurons.

Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Neuroregenerative Medicine

Target description

43-48 kDa

Physical form

Format: Purified

Protein A Purfied

Protein A Purified mouse immunoglobulin IgG1 in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.

Storage and Stability

Maintain at 2-8°C in undiluted for up to 6 months after date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Routinely evaluated by Western Blot on rat brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected GAP-43/B-50 on 10 μg of rat brain lysates.

Analysis Note

Control
Rat DRG tissue that has been subjected to a spinal nerve ligation and cultured neurons.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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