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  • MAB3494 - Anti-ATP Synthase Antibody, β chain, clone 4.3E8.D1

MAB3494 Sigma-Aldrich

Anti-ATP Synthase Antibody, β chain, clone 4.3E8.D1

clone 4.3E8.D1, Chemicon®, from mouse

Synonym: Mitochondrial Marker

  •  eCl@ss 32160702

  •  NACRES NA.41



Related Categories ATH-AZ, Alphabetical Index, Antibodies, Primary Antibodies
clone   4.3E8.D1, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  immunoprecipitation (IP): suitable
species reactivity   human, rat, mouse
shipped in   wet ice
isotype   IgG1
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_001686.3
UniProt accession no.   P06576
Gene Information   human ... ATP5B(506)



Recognizes the beta chain of ATP Synthase. The beta chain of ATP Synthase is a useful subcellular marker for mitochondria.


Epitope: beta chain

Rat brain mitochondria


Anti-ATP Synthase Antibody, β chain, clone 4.3E8.D1 detects level of ATP Synthase & has been published & validated for use in IC & IP.

Immunocytochemistry: 1 μg/mL for one hour at room temperature using 10xE6 cells or adherent sample by indirect immunofluorescence. Suggested fixative is 2-4% paraformaldehyde. Suggested permeabilization method is 0.5% Triton X-100.


Optimal working dilutions must be determined by the end user.

Research Category

Research Sub Category
Ion Channels & Transporters

Physical form

Format: Purified

Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


Antibody Basics

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