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  • MAB3494 - Anti-ATP Synthase Antibody, β chain, clone 4.3E8.D1

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MAB3494 Sigma-Aldrich

Anti-ATP Synthase Antibody, β chain, clone 4.3E8.D1

clone 4.3E8.D1, Chemicon®, from mouse

Synonym: Mitochondrial Marker

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories ATH-AZ, Alphabetical Index, Antibodies, Primary Antibodies
clone   4.3E8.D1, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  immunoprecipitation (IP): suitable
species reactivity   human, rat, mouse
shipped in   wet ice
isotype   IgG1
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_001686.3
UniProt accession no.   P06576
Gene Information   human ... ATP5B(506)

Description

Specificity

Recognizes the beta chain of ATP Synthase. The beta chain of ATP Synthase is a useful subcellular marker for mitochondria.

Immunogen

Epitope: beta chain

Rat brain mitochondria

Application

Anti-ATP Synthase Antibody, β chain, clone 4.3E8.D1 detects level of ATP Synthase & has been published & validated for use in IC & IP.

Immunocytochemistry: 1 μg/mL for one hour at room temperature using 10xE6 cells or adherent sample by indirect immunofluorescence. Suggested fixative is 2-4% paraformaldehyde. Suggested permeabilization method is 0.5% Triton X-100.

Immunoprecipitation

Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Physical form

Format: Purified

Purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
15

References

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