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  • MAB3632 - Anti-PPAR gamma 1 and 2 Antibody, phospho-specific (Ser112)

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MAB3632 Sigma-Aldrich

Anti-PPAR gamma 1 and 2 Antibody, phospho-specific (Ser112)

ascites fluid, clone, Chemicon®

Synonym: PPARgamma1 and 2

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, PP-PQL, Primary Antibodies
clone   monoclonal
biological source   mouse
application(s)   ELISA: suitable
  immunocytochemistry: suitable
  western blot: suitable
species reactivity   mouse, human
shipped in   wet ice
antibody form   ascites fluid
isotype   IgG1
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_005037.5
  NM_015869.4
  NM_138711.3
  NM_138712.3
UniProt accession no.   P37231
Gene Information   human ... PPARG(5468)

Description

Specificity

Recognizes phosphorylated Peroxisome Proliferator Activated Receptor (PPAR) gamma1 and gamma2.

Immunogen

Epitope: phospho-specific (Ser112)

Phosphorylated (serine 112) synthetic peptide from amino acids 107-121 of human PPAR gamma2 (identical to PPAR gamma1). No homology with PPAR alpha or PPAR beta.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-PPAR gamma 1 & 2 Antibody, phospho-specific (Ser112) (mouse monoclonal antibody) validated in ELISA, WB, ICC to detect PPAR gamma 1 & 2 also known as Peroxisome Proliferator Activated Receptor γ 1 & 2.

Western blot: 1:500-1:5,000 on endogenous and recombinant protein.

Immunocytochemistry: 1:500-1:5,000

ELISA: 1:500-1:5,000

Optimal working dilutions must be determined by the end user.

Physical form

Ascites. Liquid. Contains no preservative.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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