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  • MAB3684 - Anti-Cyclin B1 Antibody, clone V152

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MAB3684 Sigma-Aldrich

Anti-Cyclin B1 Antibody, clone V152

Detect Cyclin B1 with Anti-Cyclin B1 Antibody, clone V152 (Mouse Monoclonal Antibody), that has been shown to work in WB, IHC(P).

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, CT-CZ, Primary Antibodies
clone   V152, monoclonal
biological source   mouse
application(s)   immunohistochemistry: suitable (paraffin)
  western blot: suitable
species reactivity   human, mouse
shipped in   wet ice
antibody form   ascites fluid
isotype   IgG1
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_031966.2
UniProt accession no.   P14635

Description

Specificity

Cyclin B. Cyclin B1 is a marker of cell proliferation and a key component of the cell progression machinery. MAB3684 works well for detecting Cyclin B1 by Western blot and immunohistochemistry.

Immunogen

Hamster Cyclin B1.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Western blot

Immunohistochemistry (works on paraffin embedded tissue sections)

Optimal working dilutions must be determined by the end user.

Physical form

Ascites fluid. Liquid. Contains no preservative.

Storage and Stability

Maintain at -20°C in undiluted aliquots up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Linkage

Replaces: 04-220

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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