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MAB3738 Sigma-Aldrich

Anti-PML Antibody, clone 36.1-104

Anti-PML Antibody, clone 36.1-104 is a Mouse Monoclonal Antibody for detection of PML also known as Promyelocytic Leukemia Protein & has been validated in ICC, IP & WB.

Synonym: Promyelocytic Leukemia Protein, Tripartite Motif Protein 19

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Properties

Related Categories Antibodies, Primary Antibodies More...
trade name   Chemicon
brand family   Chemicon
format   Ascites
control   POSITIVE CONTROL: Mouse embryo fibroblasts (MEF1 cells).
molecular weight   106 kDa
application(s)   Immunocytochemistry
  Immunoprecipitation
  Western Blotting
isotype   IgG2b
clone   Monoclonal Antibody
  36.1-104
purification method   Unpurified
concentration   Please refer to the Certificate of Analysis for the lot-specific concentration.
host   Mouse
species reactivity   Mouse
NCBI accession no.   NM_002675.3, NM_033238.2, NM_033239.2, NM_033240.2, NM_033244.3, NM_033246.2, NM_033247.2, NM_033249.2, NM_033250.2
UniProt accession no.   P29590
gene symbol   PML(5371)
  MYL
  PP8675
  RNF71
  TRIM19
non-reactive species   Human
material size   100 µL
shipped in   wet ice
storage conditions   Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Description

Background information

PML protein is a tripartite motif (TRIM)-containing nuclear protein that may function as, among other things, a transcription factor, a coactivator of nuclear receptors (Ruggerio, 2000), a regulator of apoptosis (Guo, 2000), a mediator of interferon-induced antiviral response (Regand and Chelbi-Aliz, 2001), and as a suppressor of growth and oncogenic transformation (Mu, 1997). PML is localized to the nucleoplasm and in distinct subnuclear structures referred to as Promyelocytic Leukemia Bodies (also known as Nuclear Domain 10). Localization of PML to Promyelocytic Leukemia Bodies requires modification of PML protein by the Small Ubiquitin Modifier (SUMO) and is required for proper formation and integrity of these subnuclear structures. At least 14 splice variants of PML ranging in molecular weight from 48-97 kDa (predicted) have been described in the literature. The functional significance of the various splice variants is not well understood. In patients with Acute Promyelocytic Leukemia, the PML gene is involved in at least two specific chromosomal translocations that result in the expression of chimeric proteins with the Retinoic Acid Receptor alpha (RARalpha DNA- and Hormone-binding domains; Pandolfi, 2001). All isoforms of PML, as well as the PML-RARalpha chimeric proteins expressed in Type A and Type B APL contain an identical N-terminus but vary in the C-terminal portion of the protein (Jenson, 2001).

Physical form

Ascites fluid containing no preservatives.

Application

Western blot (1:500)

Immunoprecipitation

Immunocytochemistry (1:100)

Optimal working dilutions must be determined by end user.

Specificity

Recognizes mouse PML (Promyelocytic Leukemia protein). On western blots of protein extracts from mouse embryo fibroblast (MEF1 cells), MAB3738 recognizes a band migrating at approximately 106 kDa corresponding to PML.

Immunogen

His-tagged PML fusion protein corresponding to amino acids 1-581 of mouse PML.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Peer-Reviewed Papers
15

References

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