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  • MAB3839 - Anti-Tissue Transglutaminase Antibody, FN binding domain, clone 4G3

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MAB3839 Sigma-Aldrich

Anti-Tissue Transglutaminase Antibody, FN binding domain, clone 4G3

clone 4G3, Chemicon®, from mouse

Synonym: tTG, Protein-glutamine gamma-Glutamyltransferase, Transglutaminase 2, TGase C

  •  eCl@ss 32160702

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Properties

Related Categories Antibodies, Miscellaneous, Primary Antibodies More...
clone   4G3, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
species reactivity   human
shipped in   wet ice
Quality Level   100
mfr. no.   Chemicon®
NCBI accession no.   NM_004613.2
  NM_198951.1
UniProt accession no.   P21980
Gene Information   human ... TGM2(7052)

Description

General description

Tissue transglutaminase (tTG) is a 75 kDa monomeric globular protein expressed in the majority of cells and tissues. tTG localizes mainly in the cytoplasm but some tGT is present on the cell surface and is association with the extracellular matrix. In addition to its guanosine triphosphatase (GTPase) activity, tTG catalyzes the posttranslational modification of proteins by transamidation of available glutamine residues (Monsonego, 1997). Extracellular tTG is able to bind to and cross-link several ECM proteins and may function to stabilize the ECM and basement membranes. The protein is translated as a fully active enzyme and there is no evidence for proteolytic activation.

High constitutive expression and accumulation of active tTG is observed in (among others) endothelial cells, platelets, vascular smooth muscle cells and epithelial cells of the lens. tTG is expressed at very low levels in skeletal muscle cells and neurons and may be difficult to detect in these cell/tissue types.

Specificity

Tissue Transglutaminase. Will recognize all three known splice variants of tTG.

Immunogen

Epitope: FN binding domain

Fibronectin binding domain of tissue transglutaminase (amino acids 1-165 of tTG).

Application

Detect Tissue Transglutaminase using this Anti-Tissue Transglutaminase Antibody, FN binding domain, clone 4G3 validated for use in IC.

Immunohistochemistry: 20 μg/mL

Adhesion Blocking: 10-20 μg/mL (per 2x10e5 cells). 4G3 interferes with adhesion of TPA stimulated THP-1 cells and M-CSF stimulated peripheral blood monocytes to fibronectin.

Optimal working dilutions must be determined by the end user.



PROTOCOL:

IMMUNOCYTOCHEMISTRY: THP-1 cells were induced to differentiate into macrophages by treatment with 150 ng/ml of TPA and plated on fibronectin coated glass coverslips for 72 hours.Live non-permeablized cells were incubated with 20 μg/mL of MAB3839 for 45 minutes, washed three times with PBS and fixed for 10 minutes with 3.7% paraformaldehyde. The cells were then washed with PBS and incubated 45 minutes with a fluoresceine labeled affinity-purified goat-anti-mouse secondary antibody. After extensive washing with PBS, cells were mounted in Fluorsave medium.

Observed Staining:Cell surface tTG was colocalized with b1- and b3-Integrins in podosome-like adhesive structures of macrophages adherent on fibronectin.

Research Category
Apoptosis & Cancer

Metabolism

Research Sub Category
Apoptosis - Additional

Enzymes & Biochemistry

Physical form

Format: Purified

Protein A purified immunoglobulin in 0.02M Phosphate Buffer, pH 7.6, 0.25M NaCl containing 0.1% sodium azide.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 12 months.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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