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  • MAB3839-I - Anti-Transglutaminase-2 Antibody, FN binding domain Antibody, clone 4G3

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MAB3839-I Sigma-Aldrich

Anti-Transglutaminase-2 Antibody, FN binding domain Antibody, clone 4G3

clone 4G3, from mouse

Synonym: Protein-glutamine gamma-glutamyltransferase 2, EC: 2.3.2.13, Tissue transglutaminase, Transglutaminase C, TG(C), TGC, TGase C, Transglutaminase H, TGase H, TGase-2

  •  eCl@ss 32160702

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, TR-TR
clone   4G3, monoclonal
biological source   mouse
application(s)   flow cytometry: suitable
  immunofluorescence: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   human, human, human
packaging   antibody small pack of 25 μg
isotype   IgG1κ
antibody product type   primary antibodies
NCBI accession no.   NP_001310245
UniProt accession no.   P21980
Gene Information   human ... TGM2(7052)

Description

General description

Protein-glutamine gamma-glutamyltransferase 2 (UniProt: P21980; also known as EC: 2.3.2.13, Tissue transglutaminase, Transglutaminase C, TG(C), TGC, TGase C, Transglutaminase H, TGase H, Transglutaminase-2, TGase-2, TG2) is encoded by D13the TGM2 gene (Gene ID: 7052) in human. TG2 is a multi-domain, multi-functional enzyme that is shown to post-translationally modify proteins by catalyzing the formation of intermolecular isopeptide bonds between glutamine and lysine side-chains. It catalyes the calcium-dependent covalent modification of protein-bound glutamine side chains through transamidation or deamidation reactions. Recycling endosomes is reported to be essential for externalization of TG2, which does not follow the classical ER/Golgi-dependent secretion pathway, but is targeted to perinuclear recycling endosomes, and is delivered inside these vesicles prior to externalization. Inactivation of recycling endosomes or blocking endosome fusion with the plasma membrane is shown to block TG2 secretion. TG2 plays a critical role in extracellular matrix formation and integrin mediated signaling. It is also reported to be involved in cell signaling involving 7-transmembrane receptors. It also participates in the pathogenesis of neurodegenerative diseases and certain cancers. (Ref.: Zemskov, EA., et al (2011). PLoS ONE 6(4): e19414; Siegal, M., and Khosla, C. (2007). Pharmacol. Ther. 115(2); 232-245.

Specificity

Clone 4G3 specifically detects human Transglutaminase-2.

Immunogen

A full-length purified tissue transglutaminase (TG-2) from human erythrocytes.

Application

Anti-Transglutaminase-2, FN binding domain, clone 4G3, Cat. No. MAB3839-I, is a mouse monoclonal antibody that detects Protein-glutamine gamma-glutamyltransferase 2 and has been tested for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, and Western Blotting.

Research Category
Signaling

Western Blotting Analysis: 4 µg/mL from a representative lot detected Transglutaminase-2, FN binding domain in HUVEC lysate.

Western Blotting Analysis: A representative lot detected Transglutaminase-2, FN binding domain in Western Blotting applications (Janiak, A., et. al. (2006). Mol Biol Cell. 17(4):1606-19).

Radioimmunoassay Analysis: A representative lot detected Transglutaminase-2, FN binding domain in Radioimmunoassay applications (Akimov, S.S., et. al. (2001). Blood. 98(5):1567-76).

Flow Cytometry Analysis: A representative lot detected Transglutaminase-2, FN binding domain in Flow Cytometry applications (Zemskov, E.A., et. al. (2011). PLos One. 6(4):e19414).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Transglutaminase-2, FN binding domain in Immunoprecipitation applications (Zemskov, E.A., et. al. (2011). PLos One. 6(4):e19414; Janiak, A., et. al. (2006). Mol Biol Cell. 17(4):1606-19).

Immunofluorescence Analysis: A representative lot detected Transglutaminase-2, FN binding domain in Immunofluorescence applications (Janiak, A., et. al. (2006). Mol Biol Cell. 17(4):1606-19; Akimov, S.S., et. al. (2001). Blood. 98(5):1567-76; Zemskov, E.A., et. al. (2011). PLos One. 6(4):e19414).

Target description

~80 kDa observed; 77.33 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in PBS without azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Transglutaminase-2, FN binding domain in A431 cell lysate.

Linkage

Replaces: MAB3839

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

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