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  • MAB3872 - Anti-PPAR γ Antibody, isoform 1&2

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MAB3872 Sigma-Aldrich

Anti-PPAR γ Antibody, isoform 1&2

ascites fluid, clone 1H4, Chemicon®

Synonym: PPAR gamma

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, PP-PQL, Primary Antibodies
clone   1H4, monoclonal
biological source   mouse
application(s)   ELISA: suitable
  immunocytochemistry: suitable
  western blot: suitable
species reactivity   human, mouse
shipped in   wet ice
antibody form   ascites fluid
isotype   IgG1
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_005037.5
  NM_015869.4
  NM_138711.3
  NM_138712.3
UniProt accession no.   P37231
Gene Information   human ... PPARG(5468)

Description

General description

Peroxisome proliferator-activated receptors (PPARs) are nuclear receptors involved in lipid transport and metabolism. As such, their roles in chronic diseases such as diabetes, obesity, atherosclerosis and cancer are heavily investigated. Transcriptional activity of PPARs is regulated by fatty acid binding. Three PPAR isotypes have been identified: a, b and g. PPARg stimulates lipolysis of circulating triglycerides and the subsequent uptake of fatty acids into adipose cells. PPARs can bind to DNA only as a heterodimer with the retinoid X receptor (RXR).

Specificity

Reacts with human PPAR gamma2. The antibody also reacts with PPAR gamma1 due to immunogen sequence homology. The immunogen shows no homology with PPAR alpha or PPAR beta.

Immunogen

Epitope: isoform 1&2

Synthetic peptide from amino acids 107-121 of human PPAR gamma2.

Application

Anti-PPAR γ Antibody, isoform 1&2 is a Mouse Monoclonal Antibody for detection of PPAR gamma also known as Peroxisome Proliferator Activated Receptor γ & has been validated in ELISA, ICC & WB.

Target description

67 kDa

Analysis Note

Control
THP-1 cell lysate, 3T3-L1 cell lysate or U-937 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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