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  • MAB4146 - Anti-BCRP Antibody, clone BXP-21

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MAB4146 Sigma-Aldrich

Anti-BCRP Antibody, clone BXP-21

culture supernatant, clone BXP-21, Chemicon®

Synonym: Breast Cancer Resistance Protein, Placenta-specific ATP Binding Cassette Transporter, ATP-binding Cassette, Sub Family G, member 2 (ABCG2)

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, B-BI, Primary Antibodies
clone   BXP-21, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  immunohistochemistry: suitable (paraffin)
  immunohistochemistry: suitable
  western blot: suitable
species reactivity   human
shipped in   dry ice
antibody form   culture supernatant
isotype   IgG2a
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
input   sample type hematopoietic stem cell(s)
sample type neural stem cell(s)
NCBI accession no.   NM_004827.2
UniProt accession no.   Q9UNQ0

Description

General description

The 72 kDa breast cancer resistance protein (BCRP) is the second member of the subfamily G of the human ATP binding cassette (ABC) transporter superfamily and thus also designated as ABCG2. BCRP is a half-transporter consisting of only 1 nucleotide binding domain followed by 1 membrane-spanning domain. Current experimental evidence suggests that BCRP may function as a homodimer or homotetramer. Overexpression of BCRP is associated with high levels of resistance to a variety of anticancer agents. BCRP expression has been detected in a large number of hematological malignancies and solid tumors, indicating that this transporter may play an important role in clinical drug resistance of cancers. In addition to its role to confer resistance against chemotherapeutic agents, BCRP actively transports structurally diverse organic molecules, conjugated or unconjugated, such as estrone-3-sulfate, 17-β-estradiol 17-(β-D-glucuronide), and methotrexate. BCRP is highly expressed in the placental syncytiotrophoblasts, in the apical membrane of the epithelium in the small intestine, in the liver canalicular membrane, and at the luminal surface of the endothelial cells of human brain microvessels. This strategic and substantial tissue localization indicates that BCRP also plays an important role in absorption, distribution, and elimination of drugs that are BCRP substrates

Specificity

This antibody reacts with an internal epitope of the Breast Cancer Resistance Protein (BCRP), a 70 kDa transmembrane half-transporter which is involved in multidrug resistance. The antibody does not cross-react with human MDR1, MRP1, MRP2 or MRP5 gene products.

Immunogen

Fusion protein consisting of the E. coli maltose-binding protein and a 126-aa portion of BCRP (aa 271-396).

Application

Immunocytochemistry:
1:20 – 1:50 dilution of a previous lot was used on acetone fixed cytospin preparations.

Immunohistochemistry:
1:20 dilution of a previous lot was used on acetone fixed frozen tissue sections; 1:20 dilution of a previous lot was used on formaldehyde-fixed paraffin-embedded tissues pretreated with 10 mM citrate (pH 6.0) for 20 min.

Optimal working dilutions must be determined by end user.

Research Category
Stem Cell Research

Research Sub Category
Hematopoietic Stem Cells

This Anti-BCRP Antibody, clone BXP-21 is validated for use in IC, IH, IH(P), WB for the detection of BCRP.

Target description

70 kDa

Physical form

Mouse monoclonal IgG2a in buffer containing 0.7% BSA and 0.1% sodium azide

Unpurified

Storage and Stability

For long term storage, store at -20ºC. Stable for 6 months from date of receipt at 2-8ºC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blot on HepG2 lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected BCRP on 10 µg of HepG2 lysate.

Analysis Note

Control
MCF7 cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

WGK Germany 
WGK 2
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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