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  • MAB4150 - Anti-MRP2 Antibody, CT, clone M2 III-6

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MAB4150 Sigma-Aldrich

Anti-MRP2 Antibody, CT, clone M2 III-6

culture supernatant, clone M2 III-6, Chemicon®

Synonym: cMOAT

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, MO-MR, Primary Antibodies
clone   M2 III-6, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  immunohistochemistry: suitable (paraffin)
  immunohistochemistry: suitable
  western blot: suitable
species reactivity   human
shipped in   wet ice
antibody form   culture supernatant
isotype   IgG2a
Quality Level   300
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_000392.2
UniProt accession no.   Q92887

Description

Specificity

This antibody reacts with an internal epitope of MRP2, a 190-200 kDa transmembrane protein previously known as the canalicular multi-organic anion transporter (cMOAT). MRP2 is a member of the MRP family of multidrug resistance related proteins, and MRP2 over expression has been observed in a subset of cisplatin resistant cell lines. This antibody does not cross-react with the human MDR1, MRP1, MRP3 or MRP5 gene products.

Immunogen

Bacterial fusion protein of MRP2 containing the 202-aa C-terminal end

Epitope: C-terminus

Application

Detect MRP2 using this Anti-MRP2 Antibody, C-terminus, clone M2 III-6 validated for use in WB, IC, IH, IH(P).

Research Category
Metabolism

Research Sub Category
Toxicology & Drug Resistance

Western blotting: 1:20 - 1:50 with anti-mouse HRP

Immunocytochemistry: 1:20 - 1:50 on acetone fixed cytospin preparations

Immunohistochemistry: 1:20 on acetone fixed frozen tissue sections; 1:20 on formaldehyde-fixed paraffin-embedded tissues (pretreat with 0.01M citrate, pH 6.0, in dH2O at 100ºC for 5 minutes, repeated 3 times)

Optimal working dilutions must be determined by end user.

Physical form

Liquid containing 0.7% BSA and 0.1% sodium azide.

Storage and Stability

Maintain refrigerated at 2-8°C for up to 6 months. For long-term storage store at -20°C.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

WGK Germany 
WGK 2
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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