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  • MAB4301X - Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480, Alexa Fluor 488

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MAB4301X Sigma-Aldrich

Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480, Alexa Fluor 488

clone MC-480, Chemicon®, from mouse

Synonym: SSEA-1

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, ST-ST
conjugate   ALEXA FLUOR® 488
clone   MC-480, monoclonal
biological source   mouse
application(s)   flow cytometry: suitable
  immunocytochemistry: suitable
  immunofluorescence: suitable
species reactivity   human, mouse, rat
shipped in   wet ice
isotype   IgM
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
input   sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
sample type neural stem cell(s)
sample type: mouse embryonic stem cell(s)
NCBI accession no.   NM_002033.2
UniProt accession no.   P22083
Gene Information   human ... FUT4(2526)

Description

General description

SSEA-1 is expressed on the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.

Specificity

Reacts with the Stage-specific embryonic antigen-1 (SSEA-1) that is expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Expression of SSEA-1 is down regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is characterized by an increase in SSEA-1 expression.

Immunogen

F9 teratocarcinoma stem cells (X-irradiated)

Application

FACS

Immunocytochemistry:

1. Fix cells with a 4% Paraformaldehyde solution for 30-45 minutes, and then rinse several times with Tris-Buffered-Saline (TBS).

2. Incubate cells with MAB4301X diluted to 1/100 with PBS/ Tween-20 (0.05%) / BSA (1%) for 30 minutes.

3. Wash the sample three times with PBS / Tween-20 (0.05%) for 1 minute per wash. Examine under a microscope fitted with a FITC filter.

Optimal working dilutions must be determined by end user.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

Neural Stem Cells

This Anti-Stage-Specific Embryonic Antigen-1 Antibody, clone MC-480, Alexa Fluor 488 is validated for use in FC, IC, IF for the detection of Stage-Specific Embryonic Antigen-1.

Physical form

Ammonium Sulfate Precipitation

Purified immunoglobulin conjugated to Alexa Fluor® 488. Liquid in Phosphate buffer with 15 mg/mL BSA as a stabilizer and 0.1% sodium azide

Storage and Stability

Maintain refrigerated at 2–8°C protected from light in undiluted aliquots for up to 24 months.

Disclaimer

Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Analysis Note

Control
P19 mouse embryonic carcinoma cells

Safety & Documentation

Safety Information

WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
15

References

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