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  • MAB4334 - Anti-MDR1 Antibody, conformational extracellular epitope, clone UIC2

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MAB4334 Sigma-Aldrich

Anti-MDR1 Antibody, conformational extracellular epitope, clone UIC2

Synonym: P-glycoprotein, CD243, p170, Pgp

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, MB-ME, Primary Antibodies
clone   UIC2, monoclonal
biological source   mouse
application(s)   flow cytometry: suitable
  immunofluorescence: suitable
  immunohistochemistry: suitable (paraffin)
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
species reactivity   human, primate
should not react with   mouse, rat
shipped in   wet ice
isotype   IgG2aκ
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_000927.3
UniProt accession no.   P08183

Description

General description

UIC2 is a mouse monoclonal antibody directed against the extracellular conformational epitope of P-glycoprotein (Pgp). This antibody can be used in flow cytometry, immunofluorescence microscopy and immunohistochemistry in frozen and paraffin sections to detect Pgp expression on the membrane of MDR1 positive cells. In functional experiments, UIC2 was shown to inhibit Pgp-mediated efflux activity and potentiate the cytotoxic effects of chemotherapy drugs transported by Pgp. Additionally, the UIC2 monoclonal antibody can be used in combination with MDR1 substrates for simulatenaeous detection of Pgp expression and function by flow cytometry (MILLIPORE′s UIC2 Shift assay - see product ECM905).

Specificity

P-glycoprotein encoded by the MDR1 gene (ABCB1; LocusLink ID: 5243)

Immunogen

Epitope: conformational extracellular epitope

Mouse BALB/c 3T3 fibroblasts transfected with human MDR1 cDNA, followed by a multistep selection of tranfectants for resistance to vinblastine.

Application

Inhibition of P-glycoprotein efflux activity in functional experiments: 10 - 100 μg/mL, varies in different systems.

Immunoprecipitation: 5 - 10 fold molar excess of UIC2 over the expected Pgp concentration; realistically, 1 - 5 μg per 1 mL of cell lysate.

Flow Cytometry (conventional and UIC2 Shift assay): depends highly on Pgp expression by target cells; usually, 0.1 μg/mL for low Pgp expressors and 1 μg/mL for high Pgp expressors.

Immunofluorescence: depends highly on Pgp expression by target cells; usually, 0.1 ug/ml for low Pgp expressors and 1 μg/mL for high Pgp expressors.

IHC on frozen and paraffin sections: 1 - 10 μg/mL.

Optimal working dilutions must be determined by end user.

Research Category
Metabolism

Research Sub Category
Toxicology & Drug Resistance

Physical form

Format: Purified

The immunoglobulin was purified by protein A Sepharose® chromatography and is presented as a liquid in in 0.1M PBS, containing 0.1% sodium azide as a preservative.

Storage and Stability

Maintain at 2-8°C for up 12 months from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

Sepharose is a registered trademark of GE Healthcare

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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