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MAB4360 Sigma-Aldrich

Anti-TRA-1-60 Antibody, clone TRA-1-60

This Anti-TRA-1-60 Antibody, clone TRA-1-60 is validated for use in WB, FC, IF, IP, IC for the detection of TRA-1-60.

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, TR-TR
clone   TRA-1-60, monoclonal
biological source   mouse
application(s)   flow cytometry: suitable
  immunocytochemistry: suitable
  immunofluorescence: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   human
shipped in   wet ice
isotype   IgM
antibody product type   primary antibodies
Quality Level   100
mfr. no.   Chemicon®
input   sample type: human embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

Description

General description

Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). They express several high molecular weight glycoprotein antigens that are down-regulated upon differentiation. One of these antigens, defined by monoclonal antibody TRA-1-60, can be detected in the serum of GCT patients and provides a useful complement to the established serum markers human chorionic gonadotropin and α-fetoprotein, especially in those patients without elevated serum human chorionic gonadotropin or α-fetoprotein.

Specificity

This antibody reacts with TRA-1-60 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. Both the TRA-1-60 and TRA-1-81 monoclonal antibodies (MAB4381) recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule.

Immunogen

Human embryonal carcinoma cell line 2102Ep

Application

Immunofluorescence: A previous lot was used in IF.

Western Blot: A previous lot of this antibody was used in WB.

Immunoprecipitation: A previous lot of this antibody was used in IP.

Immunohistochemistry: A previous lot of this antibody was used in IH.

Flow Cytometry: A starting range of 10-20 µg/mL is suggested.

Optimal working dilutions must be determined by the end user.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

Target description

235/410 kDa

Physical form

Ammonium Sulfate Precipitation

Format: Purified

Purified mouse monoclonal IgM liquid in 0.05M Potassium Phosphate, 0.3M NaCl pH 8.0 with 0.05% Sodium Azide.

Storage and Stability

Stable for 1 year at from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Analysis Note

Control
NTERA-2 cl.D1 whole cell lysate (pluripotent stem cells derived from teratocarcinoma and are considered the malignant counterparts of human embryonic stem cells)

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

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HumanKine® Thermostable bFGF

Introduction Methods     – Determining stability of bFGF cytokines at elevated temperatures     – Culture of human iPS cells     – Expansion of ENStem™-A human neural progenitors Results Discussion M...
Nick Asbrock1, Christine Chen1 and Vi Chu1*

1EMD Millipore, Bioscience Division, Temecula, CA, USA
HumanZyme, Inc. Chicago, IL
Keywords: Angiogenesis, Cell biology, Cell proliferation, Degradations, Enzyme-linked immunosorbent assay, Gene expression, Growth factors, Immunocytochemistry, Immunostaining, Ligands, Microscopy, Phosphorylations, Reductions, Stem cell biology

PluriSTEM™ Human ES/iPS Medium

Current serum-free media formulations used to culture human pluripotent stem cells require daily media replenishment and at least one media exchange on the weekend. The costs in media, reagents, and ...
Min Lu1, Naomi Guyette1, Nick Asbrock1, Boris Greber2 and Vi Chu1*

1MilliporeSigma, Cellular Assays Biological Reagents & Kits, Temecula, CA, USA.
2Max Planck Institute for Molecular Biomedicine, Münster, Germany
Keywords: Autophagy, Cell culture, Cell dissociation, Evaporation, Gene expression, Polymerization reactions, Titrations, Transduction

Simplicon™ RNA Reprogramming

A ready source of induced pluripotent stem cells (iPSCs) is critical to the effective study of differentiation pathways and the investigation of the therapeutic potential of iPS cells. Since the disc...
Min Lu1, Naohisa Yoshioka2, Steven Dowdy2 and Vi Chu1*

1EMD Millipore, Bioscience Division, Temecula, CA, USA
2University of California, San Diego, La Jolla, CA, USA
Keywords: Apoptosis, Cell culture, Culture media, Gene expression, Growth factors, Microscopy, Respiratory, Transfection

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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