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  • MAB4381 - Anti-TRA-1-81 Antibody, clone TRA-1-81

MAB4381 Sigma-Aldrich

Anti-TRA-1-81 Antibody, clone TRA-1-81

Anti-TRA-1-81 Antibody, clone TRA-1-81 detects level of TRA-1-81 & has been published & validated for use in WB, FC, IF, IP & IC.

  •  eCl@ss 32160702

  •  NACRES NA.41



Related Categories Alphabetical Index, Antibodies, Primary Antibodies, TR-TR
clone   TRA-1-81, monoclonal
biological source   mouse
application(s)   flow cytometry: suitable
  immunocytochemistry: suitable
  immunofluorescence: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   human
shipped in   wet ice
isotype   IgM
antibody product type   primary antibodies
mfr. no.   Chemicon®
input   sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)


General description

Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). Embryonal carcinoma, as do all human pluripotent stem cells, expresses TRA-1-60 and TRA-1-81 antigens, and although their molecular identities are unknown, they are commonly used as markers of undifferentiated pluripotent human stem cells.


This antibody reacts with TRA-1-81 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. Both the TRA-1-81 and TRA-1-60 monoclonal antibodies (MAB4360) recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule.


Human embryonal carcinoma cell line 2102Ep


Immunofluorescence: A previous lot of this antibody was used in IF.

Western Blot: A previous lot of this antibody was used in WB.

Immunoprecipitation: A previous lot of this antibody was used in IP.

Immunohistochemistry: A previous lot of this antibody was used in IH.

Flow Cytometry: A starting range of 10-20 µg/mL is suggested.

Optimal working dilutions must be determined by the end user.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

Target description

420/250 kDa

Physical form

Ammonium Sulfate Precipitation

Format: Purified

Liquid in 0.05 M Potassium Phosphate, 0.3 M NaCl, pH 8.0 with 0.05% Sodium Azide.

Storage and Stability

Stable for 1 year at from date of receipt.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Analysis Note

Human embryonic stem cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles


Antibody Basics

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PluriSTEM™ Human ES/iPS Medium

Current serum-free media formulations used to culture human pluripotent stem cells require daily media replenishment and at least one media exchange on the weekend. The costs in media, reagents, and ...
Min Lu1, Naomi Guyette1, Nick Asbrock1, Boris Greber2 and Vi Chu1*

1MilliporeSigma, Cellular Assays Biological Reagents & Kits, Temecula, CA, USA.
2Max Planck Institute for Molecular Biomedicine, Münster, Germany
Keywords: Autophagy, Cell culture, Cell dissociation, Evaporation, Gene expression, Polymerization reactions, Titrations, Transduction

Simplicon™ RNA Reprogramming

A ready source of induced pluripotent stem cells (iPSCs) is critical to the effective study of differentiation pathways and the investigation of the therapeutic potential of iPS cells. Since the disc...
Min Lu1, Naohisa Yoshioka2, Steven Dowdy2 and Vi Chu1*

1EMD Millipore, Bioscience Division, Temecula, CA, USA
2University of California, San Diego, La Jolla, CA, USA
Keywords: Apoptosis, Cell culture, Culture media, Gene expression, Growth factors, Microscopy, Respiratory, Transfection


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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