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  • MAB5224 - Anti-Internexin α Antibody, CT, clone 2E3

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MAB5224 Sigma-Aldrich

Anti-Internexin α Antibody, CT, clone 2E3

This Anti-Internexin Antibody, α, C-terminus, clone 2E3 is validated for use in ELISA, IP, WB, IH(P) for the detection of Internexin.

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, IN-IP, Primary Antibodies
clone   2E3, monoclonal
biological source   mouse
application(s)   ELISA: suitable
  immunohistochemistry: suitable (paraffin)
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   human
species reactivity (predicted by homology)   mammals
shipped in   dry ice
antibody form   culture supernatant
isotype   IgG1
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_032727.2
UniProt accession no.   Q16352
Gene Information   human ... INA(9118)

Description

General description

The protein alpha-internexin is a ~66 kDa Class IV intermediate filament originally discovered as it copurifies with other neurofilament subunits {Pachter J et al J. Cell Biol 101:1316-22 (1985). It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed in large amounts early in neuronal development, but is downregulated in many neurons as development procedes. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M anbd NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to a-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. In addition recent studies show a marked up-regulation of a-internexin during neuronal regeneration {McGraw et al 2002}. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest.

Specificity

Alpha internexin. Localized to the C-terminal 164 amino acids of rat alpha internexin.

Immunogen

Epitope: C-terminus

Full length recombinant rat alpha internexin.

Application

Research Category
Neuroscience

Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers

Western blot: antibody detects a protein band at 55-66kDa, as expected for alpha internexin {Evans, et al, 2001}.

Immunocytochemistry on PC12 and Ntera-2 cells.

Immunohistochemistry on formalin and methanol fixed tissues. Works on paraffin embedded tissue sections.

Immunoprecipitation

ELISA

Optimal working dilutions must be determined by the end user.

Physical form

Mouse IgG in low serum (1%), tissue culture supernatant containing no preservative.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Linkage

Replaces: 04-1032

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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