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MAB5258 Sigma-Aldrich

Anti-Synaptophysin Antibody, clone SY38

clone SY38, Chemicon®, from mouse

Synonym: synaptophysin, Synaptophysin

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, SU-SY
clone   SY38, monoclonal
biological source   mouse
application(s)   flow cytometry: suitable
  immunocytochemistry: suitable
  immunohistochemistry: suitable
  western blot: suitable
species reactivity   rat, human, bovine, avian, mouse, fish
shipped in   wet ice
isotype   IgG1κ
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_003179.2
UniProt accession no.   P08247
Gene Information   human ... SYP(6855)

Description

General description

Synaptophysin is a 38 kDa glycoprotein present in the membrane of neuronal presynaptic vesicles in brain, spinal cord, retina, vesicles of adrenal medulla, neuromuscular junctions, and endocrine cells. It is also expressed by neuroendocrine cells throughout the body, both normal and neoplastic. Synaptophysin is a useful marker for the identification of normal neuroendocrine cells and neuroendocrine neoplasms. This antibody reacts with neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, and gastointestinal mucosa. It also reacts with a wide spectrum of neuroendocrine neoplasms of neural type including neuroblastomas, ganglioneuroblastomas, ganglioneuromas, pheochromocytomas, chromaffin, and non chromaffin paragangliomas.

Specificity

The antibody reacts with presynaptic vesicles of cerebral and spinal neurons, of neuromuscular endplates and with the retina of man, cow, rat and mouse. The antibody also reacts with vesicles of adrenal medulla and islet cells, and additionally allows specific staining of neuronal, adrenal and neuroepithelial tumors, such as pheochromocytoma, paraganglioma, islet cell tumors (incl. Insulinoma), medullary thyroid carcinoma and diverse pulmonary and gastro-intestinal carcinoids. The antibody also stains neurosecretory vesicles of certain culture cells, e.g. of the rat cell line PC-12.

Immunogen

Vesicular fraction of bovine brain. The SY38 epitope is to a pentapeptide repeat structure in the carboxy-terminal cytoplasmic tail of synaptophysin (Knaus & Betz 1990).

Application

Detect Synaptophysin using this Anti-Synaptophysin Antibody, clone SY38 validated for use in FC, IC, IH & WB.

Flow Cytometry (FACS):
A previous lot of this antibody was used in Flow Cytometry.

Western Blot:
(Provoda, 2000) Reacts with a 38 kDa transmembrane glycoprotein

Immunohistochemistry:
For relatively cytoplasm-rich neuroendocrinic tumors a final concentration of 1 µg/mL is recommended. Concerning cytoplasm deficient tumors a concentration of 2 µg/mL should be used. Ideal frozen sections (4-5 mm) are obtained from shock-frozen tissue samples. The frozen sections are air-dried and then fixed with acetone for 5-10 min at -20°C. Excess acetone is allowed to evaporate at room temperature. Material fixed in alcohol or formalin and embedded in paraffin can also be used.
It is advantageous to block unspecific binding sites by overlaying the sections with fetal calf serum for 20-30 min at room temperature. Excess of fetal calf serum is removed by decanting before application of the anti-body solution.
Cytocentrifuge preparations of single cells or cell smears are also fixed in acetone. These preparations should but should be followed directly by antibody treatment.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Synapse & Synaptic Biology

Target description

38 kDa

Physical form

Format: Purified

Protein A Purfied

Purified mouse monoclonal IgG1κ in buffer containing 20 mM sodium phosphate buffer, 0.25 M NaCl, containing 0.1% sodium azide.

Storage and Stability

Stable for 6 months at 2-8ºC from date of receipt. DO NOT FREEZE.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Routinely evaluated by Western Blot on mouse brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected synaptophysin on 10 μg of mouse brain lysates.

Linkage

Replaces: 04-1019

Analysis Note

Control
Positive Control: Pancreas tissue
Negative Control: Normal mouse serum
Rat hippocampus tissue, mouse brain lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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