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  • MAB5258A4 - Anti-Synaptophysin Antibody, clone SY38, Alexa Fluor 488 Conjugate

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MAB5258A4 Sigma-Aldrich

Anti-Synaptophysin Antibody, clone SY38, Alexa Fluor 488 Conjugate

clone SY38, from mouse, ALEXA FLUOR® 488

Synonym: Synaptophysin, Major synaptic vesicle protein p38

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, SU-SY
conjugate   ALEXA FLUOR® 488
clone   SY38, monoclonal
biological source   mouse
application(s)   immunohistochemistry: suitable
species reactivity   mouse, human, mouse
species reactivity (predicted by homology)   bovine (immunogen homology)
shipped in   wet ice
isotype   IgG1
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_776388
UniProt accession no.   P20488
Gene Information   human ... SYP(6855)

Description

General description

Synaptophysin is a synaptic vesicle glycoprotein with four transmembrane domains weighing 38 kDa. It is present in neuroendocrine cells and in virtually all neurons in the brain and spinal cord that participate in synaptic transmission. The localization and ubiquitous expression of this protein in neuronal tissue makes it an excellent marker for neurons and neuroendocrine tumors. The gene for this protein is located on the X chromosome (Xp11.23-p11.22). The exact function of the protein is unknown: it interacts with the essential synaptic vesicle protein synaptobrevin, but when the synaptophysin gene is experimentally inactivated in animals, they still develop and function normally.

Specificity

This antibody reacts with presynaptic vesicles of cerebral and spinal neurons, of neuromuscular endplates and with the retina of man, cow, rat and mouse. It also reacts with vesicles of adrenal medulla and islet cells.

Immunogen

Vesicular fraction of bovine brain

Application

Anti-Synaptophysin Antibody, clone SY38, Alexa Fluor 488 Conjugate detects level of Synaptophysin & has been published & validated for use in IH.

Research Category
Neuroscience

Research Sub Category
Neuronal & Glial Markers

Target description

The unconjugated parent antibody (Cat. No. MAB5258) has an observed molecular weight at ~38 kDa

Physical form

Protein G Purfied

Purified mouse monoclonal IgG1κ conjugated to Alexa Fluor® 488 in buffer containing 15 mg/ml BSA with 0.1% sodium azide.

Storage and Stability

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Quality

Evaluated by Immunohistochemistry in adult mouse brain tissue.

Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected Synaptophysin in adult mouse brain tissue.

Analysis Note

Control
Adult mouse brain tissue

Other Notes

Uncongugated antibody (MAB5258) is shown to work on Rat.

Safety & Documentation

Safety Information

WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Peer-Reviewed Papers
15

References

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