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  • MAB5258-AF555 - Anti-Synaptophysin Antibody, clone SY38, AlexaFluor 555 Conjugate

MAB5258-AF555 Sigma-Aldrich

Anti-Synaptophysin Antibody, clone SY38, AlexaFluor 555 Conjugate

clone SY38, from mouse, ALEXA FLUOR® 555

Synonym: Synaptophysin, Major synaptic vesicle protein p38, Synaptophysin

  •  eCl@ss 32160702



Related Categories Alphabetical Index, Antibodies, Primary Antibodies, SU-SY
conjugate   ALEXA FLUOR® 555
clone   SY38, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
species reactivity   rat, rat, mouse, mouse
species reactivity (predicted by homology)   avian (based on 100% sequence homology), bovine (based on 100% sequence homology), human (based on 100% sequence homology), fish (based on 100% sequence homology)
shipped in   wet ice
isotype   IgG1κ
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_776388.1
UniProt accession no.   P20488
Gene Information   human ... SYP(6855)


General description

Synaptophysin (UniProt P20488; also known as Major synaptic vesicle protein p38) is encoded by the SYP gene (Gene ID 280937) in bovine species. Synaptophysin is a 38 kDa glycoprotein present in the membrane of neuronal presynaptic vesicles in brain, spinal cord, retina, vesicles of adrenal medulla, neuromuscular junctions, and endocrine cells. It is also expressed by normal and neoplastic neural and neuroendocrine cells. Synaptophysin is a useful marker for the identification of normal neuroendocrine cells and neuroendocrine neoplasms. This antibody reacts with neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, and gastointestinal mucosa. It also reacts with a wide spectrum of neuroendocrine neoplasms of neural type including neuroblastomas, ganglioneuroblastomas, ganglioneuromas, pheochromocytomas, chromaffin, and non chromaffin paragangliomas.


The antibody reacts with presynaptic vesicles of cerebral and spinal neurons, of neuromuscular endplates and with the retina of man, cow, rat and mouse. The antibody also reacts with vesicles of adrenal medulla and islet cells, and additionally allows specific staining of neuronal, adrenal and neuroepithelial tumors, such as pheochromocytoma, paraganglioma, islet cell tumors (incl. Insulinoma), medullary thyroid carcinoma and diverse pulmonary and gastro-intestinal carcinoids. The antibody also stains neurosecretory vesicles of certain culture cells, e.g. of the rat cell line PC-12.


Epitope: The SY38 epitope is to a pentapeptide repeat structure in the carboxy-terminal cytoplasmic tail of synaptophysin (Knaus, P., and Betz, H. (1990). FEBS Lett. 261(2):358-360).

Vesicular fraction of bovine brain.


Research Category

Research Sub Category
Developmental Neuroscience

The unconjugated antibody (Cat. No. MAB5258) is shown to be suitable also for Western blotting, immunocytochemistry, and immunohistochemistry.

This Anti-Synaptophysin Antibody, clone SY38, AlexaFluor 555 Conjugate is validated for use in Immunocytochemistry for the detection of Synaptophysin.

Target description

~38 kDa observed

Physical form

Protein G Purfied

Purified mouse antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies


Evaluated by Immunocytochemistry in PC12 rat pheochromocytoma cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Synaptophysin in PC12 rat pheochromocytoma cells.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles


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