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  • MAB5280 - Anti-Tyrosine Hydroxylase Antibody, clone 2/40/15

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MAB5280 Sigma-Aldrich

Anti-Tyrosine Hydroxylase Antibody, clone 2/40/15

clone 2/40/15, Chemicon®, from mouse

Synonym: TH, Tyrosine Monooxygenase

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, TS-TZ
clone   2/40/15, monoclonal
biological source   mouse
application(s)   immunohistochemistry: suitable
  western blot: suitable
species reactivity   chicken, quail, rat, bovine
shipped in   wet ice
isotype   IgG2a
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_000360.3
UniProt accession no.   P07101

Description

General description

Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.

Specificity

Tyrosine hydroxylase (TH) catalyzes the rate-determining initial step in the biosynthesis of catecholamines such as dopamine, noradrenaline and adrenaline. Therefore the antibody is used to stain dopaminergeous and adrenergeous neurons as well as the chromaffine tissue of the adrenal medulla. Cell lines such as the PC 12 line, which is derived from a rat pheochromocytoma also, show positive reactions. In immunoblots of adrenal medulla tissue, the antibody recognizes a single protein band in the molecular weight range of 56 - 60 kD.

Immunogen

Purified tyrosine hydroxylase (EC 1.14.16.2) from a rat pheochromocytoma (2).

Application

Immunohistochemistry:
1:100-1:500. A previous lot of this antibody was used in immunohistochemistry.

Western blot:
1:1000-1:2000

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Neuronal & Glial Markers

This Anti-Tyrosine Hydroxylase Antibody, clone 2/40/15 is validated for use in IH, WB for the detection of Tyrosine Hydroxylase.

Target description

56-60 kDa

Physical form

Format: Purified

Protein A Purfied

Purified mouse monoclonal IgG2a in buffer containing 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.

Storage and Stability

Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected Tyrosine Hydroxylase on 10 μg of PC12 lysates.

Analysis Note

Control
Positive Control: Brain (sympathetic nerve terminals) and adrenal gland tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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