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MAB5492 Sigma-Aldrich

Anti-Huntingtin Antibody, a.a. 1-82

Anti-Huntingtin Antibody, a.a. 1-82 is an antibody against Huntingtin for use in ELISA, IC, IH & WB.

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, HU-HZ, Primary Antibodies
clone   2B4, monoclonal
biological source   mouse
application(s)   ELISA: suitable
  immunocytochemistry: suitable
  immunohistochemistry: suitable
  western blot: suitable
species reactivity   human
shipped in   dry ice
antibody form   ascites fluid
isotype   IgG1
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_002111.6
UniProt accession no.   P42858
Gene Information   human ... HTT(3064), SLC6A4(6532)

Description

Specificity

Reacts huntingtin protein, amino acids 1-82. The antibody recognizes wild type and mutant huntingtin.

Immunogen

Epitope: a.a. 1-82

Recombinant human huntingtin, amino acids 1-82.

Application

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Western blot: 1:500-1:5,000

Immunocytochemistry (1): 1:500-1:5,000

Immunohistochemistry (1,2): 1:500-1:5,000

ELISA: 1:500-1:5,000

Optimal working dilutions must be determined by end user.

Target description

348 kDa

Physical form

Ascites fluid containing no preservatives.

Unpurified

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Analysis Note

Control
Normal human cerebral cortex lysate, Mouse brain cortex samples from HD or wild type mice

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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