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  • MAB5556 - Anti-Nicastrin Antibody

MAB5556 Sigma-Aldrich

Anti-Nicastrin Antibody

ascites fluid, clone 9C3, Chemicon®

  •  eCl@ss 32160702

  •  NACRES NA.41



Related Categories Alphabetical Index, Antibodies, NE-NI, Primary Antibodies
clone   9C3, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   mouse, human, rat
shipped in   dry ice
antibody form   ascites fluid
isotype   IgG2b
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_015331.2
UniProt accession no.   Q92542





Synthetic peptide corresponding to the C terminal 18 a.a. of mouse Nicastrin. Due to sequence similarity, it is expected that the antibody will react with most mammals.


Anti-Nicastrin Antibody is an antibody against Nicastrin for use in IP, WB & IC.

Western blot. The antibody recognizes a doublet of ~100-110 kDa. Suggested antibody dilution buffer is TBS with 0.1% Tween-20 and 5% non-fat milk powder. Suggested incubation time is overnight at 2-8°C or 1-2 hours at room temperature.


Immunoprecipitation. Suggested tissue/cell lysis buffer is 1% Triton X100 or 2% CHAPS. Suggested final reaction volume is 1000 μL with a final protein concentration in the reaction mix of 1 mg/mL. Suggested incubation time is overnight at 2-8°C with rotating. The antibody is known to co-precipitate in CHAPS: presenilin, Aph-1 and Pen-2. Optimal working dilutions must be determined by end user.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


Antibody Basics

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Western Blot Protocol | Immunoblotting Protocol

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