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  • MAB7774 - Anti-Urokinase Antibody, NT fragment, clone 10D.7.2

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MAB7774 Sigma-Aldrich

Anti-Urokinase Antibody, NT fragment, clone 10D.7.2

clone 10D.7.2, Chemicon®, from mouse

Synonym: uPA

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, UH-UZ
clone   10D.7.2, monoclonal
biological source   mouse
application(s)   ELISA: suitable
  western blot: suitable
species reactivity   human
shipped in   wet ice
isotype   IgG2a
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_002658.2
UniProt accession no.   P00749
Gene Information   human ... PLAU(5328)

Description

Specificity

Urokinase Plasminogen Acitivator is a serine protease (SERPIN) that converts plasminogen into the active protease plasmin. uPA is secreted from cells in as the inactive 52 kDa single chain precursor which is activated into the two chain form consisting of an alpha and beta chain.MAB7774 is specific for Human uPA, other species not tested.

Immunogen

Amino Terminal Fragment of uPA

Epitope: N-terminus fragment

Application

Research Category
Metabolism

Research Sub Category
Enzymes & Biochemistry

This Anti-Urokinase Antibody, N-terminus fragment, clone 10D.7.2 is validated for use in ELISA, WB for the detection of Urokinase.

Western blot: 1:1000-1:3000

ELISA

Optimal working dilutions must be determined by end user.

Physical form

Format: Purified

Purified immunoglobulin. Liquid in 0.02 M phosphate buffer containing 0.25 M NaCl2 and 0.1% NaN3.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 6 months.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Linkage

Replaces: AB776

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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