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  • MAB8879 - Anti-Cdk4 Antibody, clone DCS-35

MAB8879 Sigma-Aldrich

Anti-Cdk4 Antibody, clone DCS-35

Detect Cdk4 with Anti-Cdk4 Antibody, clone DCS-35 (Mouse Monoclonal Antibody), that has been shown to work in IF, IP, EA, WB & IHC.

  •  eCl@ss 32160702

  •  NACRES NA.41



Related Categories Alphabetical Index, Antibodies, CDH-CDY, Primary Antibodies
clone   DCS-35, monoclonal
biological source   mouse
application(s)   activity assay: suitable
  immunofluorescence: suitable
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   rat, mouse, human, pig
shipped in   wet ice
isotype   IgG1κ
antibody product type   primary antibodies
mfr. no.   Chemicon®
NCBI accession no.   NM_000075.2
UniProt accession no.   P11802


General description

The prototype of the cdk family, p34cdc2, and a related protein cdk2, function late in the cycle while cdk4 and cdk6 are critically involved in G1 to S progression.


Recognizes a 34 kDa protein identified as p34 or cyclin-dependent kinase 4 (cdk4). It shows no cross-reaction with other cdks.


Raised against purified recombinant cdk4 protein.



Immunoprecipitation: excellent for coprecipitation of D-type cyclins bound to cdk4.

Western Blotting: 2-4 ug/mL

Kinase Assay

The prototype of the cdk family, p34cdc2, and a related protein cdk2, function late in the cycle while cdk4 and cdk6 are critically involved in G1 to S progression.

Optimal working dilutions must be determined by the end user.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Physical form

Format: Purified

Purified immunoglobulin. Liquid containing 10 mM PBS, pH 7.4 with 0.2% BSA and 0.09% sodium azide.

Storage and Stability

Maintain liquid material at 2-8°C for up to 24 months.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


Replaces: MABE548

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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