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MABC002 Sigma-Aldrich

Mouse IgG1 Negative Control, clone Ci4

Mouse IgG1 Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.

  •  eCl@ss 32160702

  •  NACRES NA.42

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Properties

Related Categories Antibody Characterization and Analysis, Biochemicals and Reagents, Isotype Controls, Proteins and Derivatives
Quality Level   100
biological source   mouse
clone   Ci4, monoclonal
mfr. no.   Chemicon®
concentration   0.5 mg/mL
application(s)   flow cytometry: suitable
  immunofluorescence: suitable
isotype   IgG1
shipped in   wet ice

Description

General description

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

Specificity

Reacts with chicken immunoglobulin. No reaction with human cell surface or plasma components has been observed. However some binding to Fc receptors may occur.

Immunogen

Chicken immunoglobulin

Application

Mouse IgG1 Negative Control Monoclonal Antibody validated for use in Flow Cytometry & Immunofluorescence.

Mouse IgG1 is a monoclonal antibody intended for use as a negative control in immunofluorescence, flow cytometry, and immunoperoxidase staining on frozen and paraffin tissue sections. Its use enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue.

SUGGESTED USAGE

The control antibody should be diluted to the same concentration as the test antibody, and equivalent volumes used.

Research Category
Secondary & Control Antibodies

Epitope Tags & General Use

Research Sub Category
Isotype Control Antibodies

Physical form

Format: Purified

Protein A Purfied

Protein A Purified mouse immunoglobulin in phosphate buffered saline, pH 7.4, containing 0.2% bovine Serum albumin and 0.1% sodium azide.

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles
Peer-Reviewed Papers
15

References

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