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MABC150 Sigma-Aldrich

Anti-LDH-A Antibody, clone 5D2.1

Anti-LDH-A, clone 5D2.1 is an antibody against LDH-A for use in WB, IH.

Synonym: Cell proliferation-inducing gene 19 protein, L-lactate dehydrogenase A chain, LDH muscle subunit, LDH-A, LDH-M, Renal carcinoma antigen NY-REN-59

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Properties

Related Categories Antibodies, Primary Antibodies More...
format   Purified
molecular weight   ~37 kDa observed
application(s)   Western Blotting
  Immunohistochemistry
isotype   IgG2aκ
clone   Monoclonal Antibody
  5D2.1
purification method   Protein G Purified
concentration   Please refer to lot specific datasheet.
host   Mouse
species reactivity   Human
NCBI accession no.   NP_001128711
UniProt accession no.   P00338
gene symbol   LDHA(3939)
  PIG19
material size   100 µg
shipped in   wet ice
storage conditions   Stable for 1 year at 2-8°C from date of receipt.

Description

Background information

L-lactate dehydrogenase A chain (LDHA), also known as Cell proliferation-inducing gene 19 protein, LDH muscle subunit, LDH-M, Renal carcinoma antigen NY-REN-59, and encoded by the gene name LDHA/ PIG19, is a member of the LDH/MDH superfamily and LDH family. Mutations in LDHA have been assoicated with Glycogen storage disease 11 (GSD11), which is a metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue.

Physical form

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Application

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected LDH-A in human breast cancer sections and human kidney tissue.

Immunogen

KLH-conjugated linear peptide corresponding to human LDH-A.

Quality Assurance

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected LDH-A in 10 µg of HeLa cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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