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  • MABC697 - Anti-ACLY, clone 5F8D11 Antibody

MABC697 Sigma-Aldrich

Anti-ACLY, clone 5F8D11 Antibody

clone 5F8D11, from mouse

Synonym: ATP-citrate synthase, ATP-citrate (pro-S-)-lyase, ACL, Citrate cleavage enzyme

  •  eCl@ss 32160702

  •  NACRES NA.41



Related Categories AC-AC, Alphabetical Index, Antibodies, Primary Antibodies
clone   5F8D11, monoclonal
biological source   mouse
application(s)   ELISA: suitable
  flow cytometry: suitable
  immunofluorescence: suitable
  immunohistochemistry: suitable
  western blot: suitable
species reactivity   mouse, monkey, human
shipped in   wet ice
isotype   IgG1
Quality Level   100
antibody product type   primary antibodies
UniProt accession no.   P53396
Gene Information   human ... ACLY(47)


General description

ATP citrate synthase, also known as ATP-citrate (pro-S-)-lyase (ACL) or Citrate cleavage enzyme, and encoded by the gene name ACLY is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine.


Purified recombinant fragment of human ACLY expressed in E. Coli


Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected ACLY in esophageal cancer tissue.

Immunofluorescence Analysis: A 1:50 dilution from a representative lot detected ACLY in HeLa cells.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected ACLY in HeLa cells.

ELISA: A 1:10,000 dilution from a representative lot was used on control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-ACLY, clone 5F8D11 Antibody is validated for use in western blotting, IHC, immunofluorescence, flow cytometry & ELISA for the detection of ACLY.

Target description

~125 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal in buffer containing PBS with up to 0.1% sodium azide and 0.5% protein stabilizer.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


Evaluated by Western Blotting in HeLa, NIH/3T3, C6, COS7, and Raji cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected ACLY in HeLa, NIH/3T3, C6, COS7, and Raji cell lysates.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


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