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  • MABC711 - Anti-ABCB5, clone 5H3C6 Antibody

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MABC711 Sigma-Aldrich

Anti-ABCB5, clone 5H3C6 Antibody

clone 5H3C6, from mouse

Synonym: ATP-binding cassette sub-family B member 5, ABCB5 P-gp, P-glycoprotein ABCB5, ABCB5

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories A1-AB, Alphabetical Index, Antibodies, Primary Antibodies
clone   5H3C6, monoclonal
biological source   mouse
application(s)   ELISA: suitable
  flow cytometry: suitable
  immunofluorescence: suitable
  immunohistochemistry: suitable
  western blot: suitable
species reactivity   human
shipped in   wet ice
isotype   IgG1
Quality Level   100
antibody product type   primary antibodies
UniProt accession no.   Q2M3G0
Gene Information   human ... ABCB5(340273)

Description

General description

ATP-binding cassette sub-family B member 5, also known as ABCB5 P-gp or P-glycoprotein ABCB5, and encoded by the gene name ABCB5, belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules. ABCB5 is responsible for the resistance to doxorubicin of a subset of malignant melanomas and is expressed in several malignant tissues.

Immunogen

Purified recombinant fragment of human ABCB5 expressed in E. Coli

Application

Immunofluorescence Analysis: A 1:200-1:1,000 dilution from a representative lot detected ABCB5 in HepG2 cells.

Flow Cytometry Analysis: A 1:200-1:400 dilution from a representative lot detected ABCB5 in HepG2 cells.

Immunohistochemistry Analysis: A 1:200-1:1,000 dilution from a representative lot detected ABCB5 in liver and breast cancer tissues.

ELISA: A representative lot was used on Control Antigen (100 ng) and Antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).

Research Category
Apoptosis & Cancer

Research Sub Category
Tumor Markers

This Anti-ABCB5, clone 5H3C6 Antibody is validated for use in western blotting, immunofluorescence, flow cytometry, IHC & ELISA for the detection of ABCB5.

Target description

~90 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1 in buffer containing PBS with up to 0.1% sodium azide and 0.5% protein stabilizer.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:500-1:2,000 dilution of this antibody detected ABCB5 in A431 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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