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  • MABC734 - Anti-phospho-ULK1 (Ser623), clone EPR6154, Rabbit Monoclonal Antibody

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MABC734 Sigma-Aldrich

Anti-phospho-ULK1 (Ser623), clone EPR6154, Rabbit Monoclonal Antibody

clone EPR6154, from rabbit

Synonym: Serine/threonine-protein kinase ULK1, ULK1

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, UH-UZ
clone   EPR6154, monoclonal
biological source   rabbit
application(s)   western blot: suitable
species reactivity   human
shipped in   wet ice
isotype   IgG
Quality Level   100
antibody product type   primary antibodies
UniProt accession no.   O75385

Description

General description

Serine/threonine-protein kinase ULK1 (ULK1) is a serine/threonine protein kinase. It is a ubiquitously expressed protein that is highly abundant in the brain, skeletal muscle, and heart. It is involved in the signaling pathway of mTOR receptors leading to the regulation of autophagy in nutrient-deprived cells. mTOR may interact with ULK1 as a complex of ULK1/FIP200/Atg13. ULK1 may play an important role in neuronal development: Elimination of ULK1′s enzymatic activity by targeted disruption of its kinase domain has blocked the outgrowth of neurons in the developing mouse brain, possibly by blocking the ULK1-mediated trafficking of neurotrophins.

Immunogen

Synthetic peptide corresponding to human phospho-ULK1 (Ser623).

Application

Anti-phospho-ULK1 (Ser623), clone EPR6154, Rabbit Monoclonal is an antibody against phospho-ULK1 (Ser623) for use in Western Blotting.

Produced in collaboration with Epitomics

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Target description

~130 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Unpurified

Rabbit monoclonal in buffer containing PBS with up to 0.1% sodium azide, 0.05% BSA, and 50% glycerol.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in ULK1 transfected HEK293 cell lysate, and ULK1 transfected HEK293 cell lysate treated with Lambda Phosphatase.

Western Blotting Analysis: A 1:1,000-10,000 dilution of this antibody detected phospho-ULK1 (Ser623) in ULK1 transfected HEK293 cell lysate, and ULK1 transfected HEK293 cell lysate treated with Lambda Phosphatase.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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