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MABC759 EMD Millipore

Anti-TSG101, clone EPR7131(B), Rabbit Monoclonal Antibody

This Anti-TSG101 antibody is validated for use in Western Blotting for the detection of TSG101.

Synonym: ESCRT-I complex subunit TSG101, TSG101, Tumor susceptibility gene 101 protein

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Properties

Related Categories Antibodies, Primary Antibodies More...
format   Unpurified
molecular weight   ~44 kDa observed. Uncharacterized bands may be observed in some lysate(s).
application(s)   Western Blotting
isotype   IgG
clone   Monoclonal Antibody
  EPR7131(B)
purification method   Unpurified
concentration   Please refer to lot specific datasheet.
host   Rabbit
species reactivity   Human, Rat, Mouse
gene symbol   TSG101(7251)
UniProt accession no.   Q99816
material size   100 µL
shipped in   wet ice
storage conditions   Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Description

Background information

Tumor susceptibility gene 101 protein (TSG101), also known as ESCRT-I complex subunit TSG101, refers to a series of cytosolic protein complexes that are needed for the proper recognition and sorting of ubiquitin modified carrier proteins into the internal vesicles of the things like endosomes, and other multivesicular bodies (MVBs). TSG101 binds to the ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into MVBs. TSG101 also mediates the association between the ESCRT-0 and ESCRT-I complex in the passage of ubiquitin cargo proteins along the chain of ESCRTs. TSG101 interacts with a protein called stathmin, a cytoplasmic phosphoprotein that is implicated in tumorigenesis, and normal TSG101 appears to act as a negative regulator of growth, genetic stability and cell cycle regulation. Mutations and alternatively spliced mutants are associated with breast and other cancers suggesting that defects in TSG101 assist in tumorigenesis and progression.

Physical form

Rabbit monoclonal in buffer containing PBS with up to 0.1% sodium azide, 0.05% BSA, and 50% glycerol.

Application

Produced in collaboration with Epitomics™

Specificity

Equilibrium disassociation constant (KD) is 7.81 x 10 -11 M

Immunogen

Synthetic peptide corresponding to human TSG101.

Quality Assurance

Evaluated by Western Blotting in PC12, NIH/3T3, HEK293, and Jurkat cell lysates.

Western Blotting Analysis: A 1:1,000-10,000 dilution of this antibody detected TSG101 in PC12, NIH/3T3, HEK293, and Jurkat cell lysates.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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