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MABC88 Sigma-Aldrich

Anti-uPAR Antibody, clone 2G10 (Azide Free)

clone 2G10, from rabbit

Synonym: Urokinase plasminogen activator surface receptor, U-PAR, uPAR, Monocyte activation antigen Mo3, CD87

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

clone   2G10, monoclonal
biological source   rabbit
application(s)   flow cytometry: suitable
  western blot: suitable
species reactivity   human
shipped in   wet ice
isotype   IgGκ
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_001005376.1
UniProt accession no.   Q03405
Gene Information   human ... PLAUR(5329)

Description

General description

UPAR protein or CD87 acts as the receptor for urokinase plasminogen activator (uPA), or urokinase, an important enzyme commonly found in the blood and extracellular matrix fluids. UPA is the enzyme that is responsible for the activation of plasmin, a powerful serine proteinase necessary for clotting, wound healing, and extracellular matrix degradation and remodeling. Urokinase also has major roles in vascular disease and cancer growth and spreading. UPAR, the receptor, is also important in cancer biology as it is through this receptor that the uPA effects are mediated, and these effects play a role in cell adhesion, migration and cell proliferation of cancers. UPAR also binds vitronectin and uPAR associated proteins (uPARAP) as well as several members of the integrin family of proteins. UPAR’s primary physiological role is as part of the uPA activation system which is involved in tissue reorganization and wound healing. By binding urokinase it restricts the activation of plasminogen to plasmin to the immediate vicinity of the cell membrane and thus helps to regulate and limit the strength and duration of the remodeling process.

Specificity

This antibody competes against urokinase plasminogen activator (uPA) for uPAR binding. Inhibition of uPAR is maximized with the combination of Anti-uPAR, clone 3C6 (Cat. # MABC89) and Anti-uPAR, clone 2G10 (Cat. # MABC88).

Application

This Anti-uPAR Antibody, clone 2G10 (Azide Free) is validated for use in Western Blotting and Flow Cytometry and Inhibits Activity/Function for the detection of uPAR.

Target description

~45 kDa observed.
Recombinant human uPAR (Leu23-Arg303) has a predicted molecular weight of ~31 kDa, but runs around ~45 kDa under non-reduced conditions.

Physical form

Format: Purified

Purified rabbit monoclonal IgGκ in buffer containing PBS without preservatives.

Quality

Evaluated by Western Blotting in reduced and non-reduced human uPAR recombinant protein.

Western Blotting Analysis: A 1:2500 dilution of this antibody detected uPAR in 1 µg of non-reduced human uPAR recombinant protein, but not in 1 µg of reduced human uPAR recombinant protein.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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