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MABC89 Sigma-Aldrich

Anti-uPAR Antibody, clone 3C6 (Azide Free)

clone 3C6, from rabbit

Synonym: Urokinase plasminogen activator surface receptor, U-PAR, uPAR, Monocyte activation antigen Mo3, CD87

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, UH-UZ
clone   3C6, monoclonal
biological source   rabbit
application(s)   flow cytometry: suitable
  western blot: suitable
species reactivity   human
shipped in   wet ice
isotype   IgG1λ
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_001005376
UniProt accession no.   Q03405
Gene Information   human ... PLAUR(5329)

Description

General description

UPAR protein or CD87 acts as the receptor for urokinase plasminogen activator (uPA), or urokinase, an important enzyme commonly found in the blood and extracellular matrix fluids. UPA is the enzyme that is responsible for the activation of plasmin, a powerful serine proteinase necessary for clotting, wound healing, and extracellular matrix degradation and remodeling. Urokinase also has major roles in vascular disease and cancer growth and spreading. UPAR, the receptor, is also important in cancer biology as it is through this receptor that the uPA effects are mediated, and these effects play a role in cell adhesion, migration and cell proliferation of cancers. UPAR also binds vitronectin and uPAR associated proteins (uPARAP) as well as several members of the integrin family of proteins. UPAR’s primary physiological role is as part of the uPA activation system which is involved in tissue reorganization and wound healing. By binding urokinase it restricts the activation of plasminogen to plasmin to the immediate vicinity of the cell membrane and thus helps to regulate and limit the strength and duration of the remodeling process.

Specificity

This antibody competes with beta 1 integrins for uPAR binding and inhibits uPAR-mediated cell invasion. Inhibition of uPAR is maximized with the combination of Anti-uPAR, clone 3C6 (Cat. # MABC89) and Anti-uPAR, clone 2G10 (Cat. # MABC88).

Immunogen

Linear peptide corresponding to Human uPAR.

Application

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-uPAR Antibody, clone 3C6 (Azide Free) is validated for use in Western Blotting and Flow Cytometry and Inhibits Activity/Function for the detection of uPAR.

Western Blot Analysis: A representative lot of this antibody detected non-reduced uPAR recombinant protein (see Harel, E., et al., 2014 UCSF Scientific Poster).

Flow Cytometry Analysis: A representative lot of this antibody detected uPAR in MDA-MB-231, H1299 and PC3 cells (see Harel, E., et al., 2014 UCSF Scientific Poster).

Flow Cytometry Analysis: A representative lot of this antibody detected uPAR in HEK293 cells stably expressing uPAR (Dureseti et al., (2010) JBC. 285(35):26878–26888).

Inhibition Assay: A representative lot of this antibody inhibted the invasion of MDA-MB-231 cells (see Harel, E., et al., 2014 UCSF Scientific Poster). Inhibition of uPAR is maximized with the combination of Anti-uPAR, clone 3C6 (Cat. # MABC89) and Anti-uPAR, clone 2G10 (Cat. # MABC88).

Inhibition Assay: A representative lot of this antibody blocked uPAR-mediated invasion and signaling in H1299 cells (Dureseti et al., (2010) JBC. 285(35):26878–26888). Inhibition of uPAR is maximized with the combination of Anti-uPAR, clone 3C6 (Cat. # MABC89) and Anti-uPAR, clone 2G10 (Cat. # MABC88).

Target description

~45 kDa observed.
Recombinant human uPAR (Leu23-Arg303) has a predicted molecular weight of ~31 kDa, but runs around ~45 kDa under non-reduced conditions.

Physical form

Format: Purified

Protein A Purfied

Purified rabbit monoclonal IgGλ in buffer containing PBS without preservatives.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in reduced and non-reduced human uPAR recombinant protein.

Western Blotting Analysis: A 1:2500 dilution of this antibody detected uPAR in 1 µg of non-reduced human uPAR recombinant protein, but not in 1 µg of reduced human uPAR recombinant protein.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

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Protocols

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Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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