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MABD102 Sigma-Aldrich

Anti-Lin28 Antibody, clone PR3G10

Anti-Lin28 Antibody, clone PR3G10 is a highly specific mouse monoclonal antibody, that targets lin28 & has been tested in western blotting & ICC.

Synonym: Lin-28A, Protein lin-28 homolog A, Zinc finger CCHC domain-containing protein 1

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Properties

Related Categories Antibodies, Primary Antibodies More...
format   Purified
control   Human placenta tissue lysate
molecular weight   ~28 kDa observed
application(s)   Western Blotting
  Immunocytochemistry
isotype   IgG1κ
clone   Monoclonal Antibody
  PR3G10
purification method   Protein G Purified
epitope   Near N-Terminus
concentration   Please refer to the Certificate of Analysis for the lot-specific concentration.
host   Mouse
species reactivity   Human
NCBI accession no.   NP_078950
UniProt accession no.   Q9H9Z2
gene symbol   LIN28A(79727)
  CSDD1
  LIN28
  ZCCHC1
material size   100 µg
shipped in   wet ice
storage conditions   Stable for 1 year at 2-8°C from date of receipt.

Description

Background information

Lin28 is a developmentally regulated RNA binding protein (RBP). Its down regulation is mediated in part by Lin4 and miR-125b, the mammalian micro-RNA homolog of Lin4. It is known to be negatively regulated by retinoic acid and act as a translational enhancer by driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis by increasing the number of initiation events per mRNA molecule. Lin28 is necessary and sufficient for selectively blocking the cleavage processing of pri-let-7 miRNAs in embryonic cells. Lin28 is a negative regulator of miRNA biogenesis and may play a role in blocking miRNA-mediated differentiation in stem cells and in certain cancers. The mechanism by which miR-125b represses Lin28 in mammalian cells appears to involve reductions not only in translational efficiency, but also in mRNA abundance. Lin28 has shown to be essential for skeletal muscle differentiation through the translational upregulation of IGF2 expression. It is known to be expressed in testis, placenta, and embryonic stem (ES) cells.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide.

Application

Western Blot Analysis: 1 µg/mL from a representative lot detected Lin28 in human fetal brain tissue lysate.

Immunocytochemistry Analysis: A 1:500 dilution (2 µg/mL) from a representative lot detected Lin28 in mESCs.

Immunogen

Ovalbumin-conjugated linear peptide corresponding to human Lin28.

Quality Assurance

Evaluated by Western Blot in human placenta tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Lin28 in 10 µg of human placenta tissue lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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