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  • MABD102 - Anti-Lin28 Antibody, clone PR3G10

MABD102 Sigma-Aldrich

Anti-Lin28 Antibody, clone PR3G10

clone PR3G10, from mouse

Synonym: Protein lin-28 homolog A, Lin-28A, Zinc finger CCHC domain-containing protein 1

  •  eCl@ss 32160702

  •  NACRES NA.41



Related Categories Alphabetical Index, Antibodies, L-LL, Primary Antibodies
clone   PR3G10, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  western blot: suitable
species reactivity   human
shipped in   wet ice
isotype   IgG1κ
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_078950
UniProt accession no.   Q9H9Z2
Gene Information   human ... LIN28A(79727)


General description

Lin28 is a developmentally regulated RNA binding protein (RBP). Its down regulation is mediated in part by Lin4 and miR-125b, the mammalian micro-RNA homolog of Lin4. It is known to be negatively regulated by retinoic acid and act as a translational enhancer by driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis by increasing the number of initiation events per mRNA molecule. Lin28 is necessary and sufficient for selectively blocking the cleavage processing of pri-let-7 miRNAs in embryonic cells. Lin28 is a negative regulator of miRNA biogenesis and may play a role in blocking miRNA-mediated differentiation in stem cells and in certain cancers. The mechanism by which miR-125b represses Lin28 in mammalian cells appears to involve reductions not only in translational efficiency, but also in mRNA abundance. Lin28 has shown to be essential for skeletal muscle differentiation through the translational upregulation of IGF2 expression. It is known to be expressed in testis, placenta, and embryonic stem (ES) cells.


Epitope: Near N-Terminus

Ovalbumin-conjugated linear peptide corresponding to human Lin28.


Anti-Lin28 Antibody, clone PR3G10 is a highly specific mouse monoclonal antibody, that targets lin28 & has been tested in western blotting & ICC.

Research Category
Stem Cell Research

Research Sub Category
RNA Metabolism & Binding Proteins

Western Blot Analysis: 1 µg/mL from a representative lot detected Lin28 in human fetal brain tissue lysate.

Immunocytochemistry Analysis: A 1:500 dilution (2 µg/mL) from a representative lot detected Lin28 in mESCs.

Target description

~28 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


Evaluated by Western Blot in human placenta tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Lin28 in 10 µg of human placenta tissue lysate.

Analysis Note

Human placenta tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles


Antibody Basics

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