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MABD407 Sigma-Aldrich

Anti-SIP1 Antibody, clone 6E5

Anti-SIP1 Antibody, clone 6E5 is an antibody against SIP1 for use in western blotting, ICC & IHC.

Synonym: Smad-interacting protein 1, SMADIP1, Zinc finger homeobox protein 1b, Zinc finger E-box-binding homeobox 2, SIP1

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, SG-SK
clone   6E5, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  immunohistochemistry: suitable
  western blot: suitable
species reactivity   human
shipped in   wet ice
isotype   IgG2aκ
antibody product type   primary antibodies
concentration   1 mg/mL
NCBI accession no.   NP_001165124
UniProt accession no.   O60315
Gene Information   human ... ZEB2(9839)

Description

General description

Smad-interacting protein 1 (SIP1, SMADIP1), also known as Zinc finger E-box-binding homeobox 2 (ZEB2), and Zinc finger homeobox protein 1b (ZFHX1B, ZFX1B), is a transcriptional inhibitor that binds to DNA sequence 5′-CACCT-3′ in different promoters and interacts with activated SMADs. ZEB2 has been shown to repress the transcription of the E-cadherin gene and induce epithelial-mesenchymal transition, a genetic program controlling cell migration during embryonic development and wound healing in vitro. It is suggested that ZEB2 contributes to tumor progression since it’s been demonstrated to protect cells from DNA damage-induced apoptosis. Recent studies have shown that ZEB2 is often observed in the cytoplasm in numerous cancer tissues, implying that its localization may be regulated in normal and tumor tissues. Mutations in this gene are also associated with Mowat-Wilson syndrome (MWIS), also known as Hirschsprung disease-mental syndrome.

Immunogen

Epitope: N-terminus

His-tagged recombinant protein corresponding to the N-terminus of Human SIP1.

Application

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

This antibody has been shown to work in Immunocytochemistry and Immunohistochemistry applications (Oztas et al., 2010).

Target description

~220 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blot in a SK-Hep1 cell lysate. 2ug/ml (1:500 dilution) of this antibody detected SIP-1 in a SK-Hep1 cell lysate.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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