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  • MABE1124 - Anti-XPA Antibody, clone 1XPA Antibody-1E11

MABE1124 Sigma-Aldrich

Anti-XPA Antibody, clone 1XPA Antibody-1E11

ascites fluid, clone 1XPA-1E11, from mouse

Synonym: DNA repair protein complementing XP-A cells, XPA, Xeroderma pigmentosum group A-complementing protein

  •  eCl@ss 32160702

  •  NACRES NA.41



Related Categories Alphabetical Index, Antibodies, Primary Antibodies, X
clone   1XPA-1E11, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  western blot: suitable
species reactivity   human
shipped in   dry ice
antibody form   ascites fluid
isotype   IgG1κ
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_000371
UniProt accession no.   P23025


General description

DNA repair protein complementing XP-A cells (UniProt P23025; also known as Excision repair-controlling, Mutant xeroderma pigmentosum complementation group A, Xeroderma pigmentosum group A-complementing protein) is encoded by the XPA (also known as XP1, XPAC) gene (Gene ID 7507) in human. DNA lesions caused by UV irradiation, drugs, or other environmental factors are eliminated by two nucleotide excision repair (NER) pathways, Global genome repair (GGR) and transcription-coupled repair (TCR). In GGR, the removal of lesions requires their recognition by the repair factor XPC/HR23b and the subsequent opening of the DNA duplex by transcription factor II human (TFIIH). The resulting single-stranded structure is stabilized by XPA and replication protein A (RPA). XPG is recruited through its interaction with TFIIH on the 3′ side of the lesion and its positioning on the cut site requires RPA. The interaction between XPA and XPB (ERCC1) stimulates the recruitment of ERCC1-XPF on the 5′ side of the DNA lesion. The damaged oligonucleotide can then be removed through the double incision by XPG and ERCC1-XPF endonucleases. In TCR, these factors (except XPC/HR23B) are recruited by the stalled RNA pol II in front of the damage with the help of the CSB and CSA proteins.


Epitope: Near C-terminus

Linear peptide corresponding to human XPA sequence near C-terminus.


Anti-XPA Antibody, clone 1XPA Antibody-1E11 is an antibody against XPA for use in Western Blotting, Immunocytochemistry.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Western Blotting Analysis: A representative lot detected the ATP-dependent recruitment of XPA in HeLa nuclear extract onto the stalled RNA pol IIO on mono-cisplatin-damaged DNA (Riedl, T., et al. (2003). EMBO J. 22(19):5293-5303; Laine, J.P., and, Egly, J.M. (2006). EMBO J. 25(2):3873-97).
Immunocytochemistry Analysis: A representative lot detected XPA recruitment to the DNA damage sites in the nuclei of UV-irradated HeLa cells (Alekseev, S., et al. (2014). Chem Biol. 21(3):398-407).

Target description

~37 kDa observed. Target band appears larger than the calculated molecular weight of 31.4 kDa, most likely due to posttranslational modifications. Uncharacterized band(s) may appear in some lysates.

Physical form

Mouse monoclonal IgG1κ ascites with 0.05% sodium azide.


Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected XPA in 10 µg of HeLa nuclear extract.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles


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