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  • MABE1669 - Anti-Cas9 Antibody, NT Antibody, clone 8C1-F10

MABE1669 Sigma-Aldrich

Anti-Cas9 Antibody, NT Antibody, clone 8C1-F10

clone 8C1-F10, from mouse

Synonym: CRISPR-associated endonuclease Cas9/Csn1, SpyCas9

  •  eCl@ss 32160702



Related Categories Alphabetical Index, Antibodies, CAM-CB, Primary Antibodies
clone   8C1-F10, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  western blot: suitable
species reactivity   Streptococcus pyogenes, Streptococcus pyogenes
species reactivity (predicted by homology)   all
shipped in   ambient
isotype   IgG2bκ
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_269215
UniProt accession no.   Q99ZW2


General description

CRISPR-associated endonuclease Cas9/Csn1 (UniProt Q99ZW2; also known as SpyCas9) is encoded by the Streptococcus pyogenes serotype M1 gene Cas9 (also known as csn1) gene (Gene ID 901176). Clustered regularly interspaced short palindromic repeats (CRISPRs) are DNA loci containing short repetitions of base sequences complementary to antecedent mobile elements and target invading nucleic acids. Each repetition is followed by short segments of spacer DNA from previous exposures to a virus. Cas9 is an essential endonuclease in Streptococcus pyogenes serotype M1′s CRISPR immune system that confers resistance to foreign genetic elements such as plasmids and phages. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems, correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc), and Cas9. Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, and then trimmed 3′-5′ exonucleolytically.


Clone 8C1-F10 targets an epitope within Cas9 N-terminal region outside the active site RuvC-I.


His-tagged recombinant Cas9 N-terminal fragment excluding the active site RuvC-I.


Anti-Cas9, NT, clone 8C1-F10 , Cat. No. MABE1669, is a highly specific mouse monoclonal antibody that targets CRISPR-associated endonuclease Cas9/Csn1 and has been tested in Immunocytochemistry and Western Blotting.

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected exogenously expressed full-length nuclease-dead Cas9 in transfected NIH/3T3 cells fixed with 4% paraformaldehyde and permeabilized by 0.3% Triton X-100.

Research Category
Epigenetics & Nuclear Function

Target description

~160 kDa observed. 158.4 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified.

Purified mouse IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


Evaluated by Western Blotting in NIH3T3 cells.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected exogenously expressed full-length nuclease-dead Cas9 in 10 µg of lysate from transfected NIH/3T3 cells.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles


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