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MABE1671M Sigma-Aldrich

Anti-CCAR1 Antibody, clone 4B9.1

Anti-CCAR1, clone 4B9.1, Cat. No. MABE1671, is a highly specific mouse monoclonal antibody, that targets Cell division cycle and apoptosis regulator protein 1 and has been tested in Western Blotting and Immunocytochemistry.

Synonym: CARP-1, Cell cycle and apoptosis regulatory protein 1, Cell division cycle and apoptosis regulator protein 1, Death inducer with SAP domain

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Properties

Related Categories Antibodies, Primary Antibodies More...
format   Purified
molecular weight   135 kDa observed. Uncharacterized bands may be observed in some lysate(s).
application(s)   Western Blotting
  Immunocytochemistry
isotype   IgG2b
clone   Monoclonal Antibody
purification method   Protein G purified
concentration   Please refer to lot specific datasheet.
host   Mouse
species reactivity   Human
gene symbol   CCAR1(55749)
  CARP1
  DIS
UniProt accession no.   Q8IX12
material size   25 μg
shipped in   ambient
storage conditions   Stable for 1 year at 2-8°C from date of receipt.

Description

Background information

Cell division cycle and apoptosis regulator protein 1 (UniProt: Q8IX12; also known as Cell cycle and apoptosis regulatory protein 1, CARP-1, Death inducer with SAP domain, CCAR1) is encoded by the CCAR1 (also known as CARP1, DIS) gene (Gene ID: 55749) in human. CCAR1 is a cytoplasmic protein that localized to the perinuclear region. It is expressed in various epithelial cancer cell lines, including breast, colon, prostate, pancreatic and leukemia. Its expression is shown to be regulated by growth factors. Two isoforms of CCAR1 have been reported that are generated by alternative splicing. CCAR1 associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. CCAR1 is recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. It also functions as a p53 coactivator and may play a role in transcriptional regulation. It is reported to be involved in apoptosis signaling in the presence of the reinoid CD437. In association with CALCOCO1, it enhances GATA1- and MED1-mediated transcriptional activation from the gamma-globin promoter during erythroid differentiation of K562 erythroleukemia cells. CCAR1 can act as a coactivator and corepressor of AR-mediated transcription and contribute to chromatin looping and AR transcription complex assembly by stabilizing AR-GATA2 association on chromatin and facilitating MED1 and RNA polymerase II recruitment to AR-binding sites.

Physical form

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Application

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected CCAR1 in HeLa, A431, and HUVEC cells.
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected CCAR1 in 10 µg of  HepG2 cell lysate.

Specificity

Clone 4B9.1 detects Cell division cycle and apoptosis regulator protein 1 (CCAR1) in human cells. It targets an epitope witihin 173 amino acids from the N-terminal half.

Immunogen

GST/His-tagged recombinant fragment corresponding to 173 amino acids from the N-terminal half of human Cell division cycle and apoptosis regulator protein 1 (CCAR1).

Species Reactivity Notes

Human.

Quality Assurance

Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected CCAR1 in 10 µg of HeLa cell lysate.

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles
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