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  • MABE1795 - Anti-PP4C Antibody, clone 7B5

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MABE1795 Sigma-Aldrich

Anti-PP4C Antibody, clone 7B5

clone 7B5, from mouse

Synonym: Serine/threonine-protein phosphatase 4 catalytic subunit, EC: 3.1.3.16, Pp4, PPP4C, Protein phosphatase X, PP-X

  •  eCl@ss 32160702

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Properties

Related Categories Alphabetical Index, Antibodies, PP-PQL, Primary Antibodies
clone   7B5, monoclonal
biological source   mouse
application(s)   western blot: suitable
species reactivity   human, human
species reactivity (predicted by homology)   bovine (based on 100% sequence homology), rat (based on 100% sequence homology), rabbit (based on 100% sequence homology)
packaging   antibody small pack of 25 μL
isotype   IgG1κ
antibody product type   primary antibodies
NCBI accession no.   NP_002711.1
UniProt accession no.   P60510
Gene Information   human ... PPP4C(5531)

Description

General description

Serine/threonine-protein phosphatase 4 catalytic subunit (UniProt: P60510; also known as EC: 3.1.3.16, PP4C, Pp4, Protein phosphatase X, PP-X) is encoded by the PPP4C (also known as PPP4, PPX) gene (Gene ID: 5531) in human. Protein phosphatase 4 (PP4), a ubiquitous serine/threonine phosphatase, is involved in the regulation of many cellular processes. It functions independently of protein phosphatase 2A (PP2A). Mammalian PP4 contains two regulatory subunits (R1 and R2) that interact with the catalytic subunit (PP4C)) and control its activity. Methylation at the C-terminal Leucine 307 is critical for interactions with regulatory subunits. PP4C-R2 complexes are reported to be involved in organelle assembly, maturation of the centrosome, apoptosis, DNA repair, and spliceosomal assembly. It is also reported to play a role in cell migration and in activation of c-Jun N-terminal kinase and NF-kappa B. PP4C has multiple metal ion binding sites and can bind two manganese ions per subunit. (Ref.: Cohen, PTW., et al. (2005). FEBS Lett. 579(15); 3278-3286).

Specificity

Clone 7B5 specifically detects human Serine/threonine-protein phosphatase 4 catalytic subunit (PP4C). It targets an epitope within 17 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide coreesponding to 17 amino acids from the C-terminal region of human Serine/threonine-protein phosphatase 4 catalytic subunit (PP4C).

Application

Anti-PP4C, clone 7B5, Cat. No. MABE1795, is a highly specific mouse monoclonal antibody that targets Serine/threonine-protein phosphatase 4 catalytic subunit and has been tested for use in Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: A 1:500 dilution from a representative lot detected PP4C in lysate from NIH3T3 mouse fibroblasts ectopically expressing N-terminally tagged human PP4 catalytic subunit (PPP4C).(Courtesy of Dr. Stefan Schuechner and Dr. Egon Ogris, Medical University of Vienna, Austria).

Target description

35.08 kDa calculated.

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

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