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MABE339 Sigma-Aldrich

Anti-p53 (wild type) Antibody, clone PAb1620

Anti-p53 (wild type) Antibody, clone PAb1620 is a Mouse Monoclonal Antibody for detection of p53 (wild type) also known as Cellular tumor antigen p53, Tumor suppressor p53 & has been validated in WB & IHC.

Synonym: Cellular tumor antigen p53, Tumor suppressor p53

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, P-PA, Primary Antibodies
clone   Pab1620, monoclonal
  monoclonal
biological source   mouse
application(s)   immunohistochemistry: suitable
  western blot: suitable
packaging   100 µg (MABE339)
NCBI accession no.   NP_001120705
UniProt accession no.   P02340
isotype   IgG2aκ
shipped in   wet ice

Description

General description

Wild-type p53 is present in the nucleus of all normal mammalian cells where it appears to be involved in the regulation of cell proliferation. The normal protein has a very short half-life and is present in only minute amounts in normal tissues and cells. In contrast, mutant p53 protein produced by malignant cells is usually a product of a point mutation in the p53 gene leading to substitution of a single amino acid that significantly prolongs the half-life of the protein. The accumulation of high levels of p53 is a potential novel marker for malignancy.

Immunogen

Mouse VLM tumor cells.

Application

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected p53 in normal human brain tissue.

Immunoprecipitation Analysis: A representative lot from an independent laboratory detected p53 in IP (Lu, X., et al. (1992). Cell. 70(1):153-161.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected p53 in dorsal skin samples from animal trunks (Kramata, P., et al. (2005). Cancer Res. 65(9):3577-3585.) and in certain human cancer tissues (Cordon-Cardo, C., et al, (1991). 51(23 Pt 1):6372-6380.).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Target description

~53 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Analysis Note

Control
Mouse brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected p53 in mouse brain tissue lysate.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Peer-Reviewed Papers
15

References

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