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  • MABE354 - Anti-Uracil-DNA glycosylase Antibody, clone 8G10.1

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MABE354 Sigma-Aldrich

Anti-Uracil-DNA glycosylase Antibody, clone 8G10.1

clone 8G10.1, from mouse

Synonym: UDG, Uracil-DNA glycosylase

  •  eCl@ss 32160702

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, UH-UZ
clone   8G10.1, monoclonal
biological source   mouse
application(s)   immunohistochemistry: suitable
  western blot: suitable
species reactivity   human, human
species reactivity (predicted by homology)   chimpanzee (based on 100% sequence homology), monkey (based on 100% sequence homology)
shipped in   wet ice
isotype   IgG2aκ
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_003353
UniProt accession no.   P13051

Description

General description

Uracil-DNA glycosylase (EC 3.2.2.27; UniProt P13051; also known as UDG) is encoded by the UNG (also known as DGU, HIGM4, HIGM5, UNG1, UNG2, UNG15) gene (Gene ID 7374) in human. Uracil-DNA glycosylases catalyze the release of uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway that plays an important role in mutagenesis prevention. Uracil bases in DNA can result from cytosine deamination or misincorporation of dUMP instead of dTTP during DNA synthesis. Alternative promoter usage and splicing of UNG gene lead to two different isoforms, the mitochondrial UNG1 and the nuclear UNG2. The mammalian shelterin complex contains the six proteins (TRF1, TRF2, RAP1, TIN2, POT1, and TPP1), the complex occupies the end of the telomeres and protect telomeres against inappropriate DNA repair when telomeres are not being lengthened. UNG deficiency in primary mouse hematopoietic cells is shown to affect POT1 (Protector of Telomeres 1) recognition of the telomere repeats due to increased uracil incorporation in telomeres TTAGGG repeats, which in turn leads to abnormal telomere lengthening.

Specificity

Expected to react with both spliced isoforms (UNG1 & UNG2) of human Uracil-DNA glycosylase.

Immunogen

Epitope: Near C-terminus

Linear peptide corresponding to human Uracil-DNA glycosylase near the C-terminus.

Application

Anti-Uracil-DNA glycosylase Antibody, clone 8G10.1, Cat. No. MABE354, is a highly specific mouse monoclonal antibody, that targets UNG and has been tested in Western Blotting and Immunohistochemistry.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Uracil-DNA glycosylase in human skeletal muscle and human cardiac tissues.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Target description

~34 kDa observed (33.9 kDa/UNG1 & 34.6 kDa/UNG2 calculated). Immunogen sequence should allow to detect both isoform 1 and 2. Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Uracil-DNA glycosylase in 10 µg of HepG2 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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