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MABE56 Sigma-Aldrich

Anti-pan Ago Antibody, clone 2A8

Anti-pan Ago Antibody, clone 2A8 is a Mouse Monoclonal Antibody for detection of pan Ago also known as protein argonaute-2 or PAZ Piwi domain protein & has been validated in WB, IP, ICC & IHC.

Synonym: eukaryotic translation initiation factor 2C, 2, protein argonaute-2, argonaute 2, Protein slicer, argonaute2, PAZ Piwi domain protein, Eukaryotic translation initiation factor 2C, Argonaute2, Argonaute4, Ago4, Protein argonaute-4

  •  eCl@ss 32160702

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Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   100 µg (MABE56-25UG)
  25 μg (MABE56-25UG)
application(s)   immunocytochemistry: suitable
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
biological source   mouse
clone   2A8, monoclonal
isotype   IgG1κ
shipped in   ambient
species reactivity   human
gene symbol   AGO1(26523)
  AGO2(27161)
  AGO3(192669)
format   Purified
molecular weight   ~ 95 and 70 kDa observed. This antibody recognizes Ago1, Ago2, Ago3, and Ago4.
Note that this antibody has been reported to cross-react with radixin (~70 kDa). (Nelson, P., et al. (2007). RNA. 13:1787–1792.)
clone   Monoclonal Antibody
concentration   Please refer to the Certificate of Analysis for the lot-specific concentration.
gene symbol   : AGO4
 
EIF2C4
  Ago1
  Argonaute1
  Argonaute3
  EIF2C1
  EIF2C2l
  EIF2C3
  KIAA1567
  PPD
  Q10
  eIF-2C 1
  eIF-2C 2
  hAgo2
Quality Assurance   Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Ago on 10 µg of HeLa cell lysate.

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Target description

~ 95 and 70 kDa observed. This antibody recognizes Ago1, Ago2, Ago3, and Ago4.
Note that this antibody has been reported to cross-react with radixin (~70 kDa). (Nelson, P., et al. (2007). RNA. 13:1787–1792.)

Analysis Note

Control
HeLa cell lysate

Application

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Nelson, P., et al. (2007). RNA. 13:1787–1792.)

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC. (Nelson, P., et al. (2007). RNA. 13:1787–1792.)

Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in IH. (Nelson, P., et al. (2007). RNA. 13:1787–1792.)

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

RNA Metabolism & Binding Proteins

General description

Argonaute (Ago) proteins are a family of proteins of ~95 kDa that bind directly to mature miRNAs. In mammals, there are four Ago proteins (Ago1-4). This family of proteins is a central component of most mammalian miRNPs that mediates important functions in small RNA-directed regulatory pathways. The Ago family consists of two subclases, Ago and Piwi containing two characteristic domains known as PAZ and PIWI. These two domains interact with different parts of the mature miRNA. The PAZ domain interacts with the 3’-end of the miRNA, and the PIWI domain interacts with the 5’ phosphate and 5’-proximal region of the miRNA to guide target mRNA recognition.

Immunogen

Histidine - tagged recombinant protein corresponding to human Ago.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Protein G

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Peer-Reviewed Papers
15

References

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