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MABE901 Sigma-Aldrich

Anti-TAF15 Antibody, clone 4D71

clone 4D71, from mouse

Synonym: TATA-binding protein-associated factor 2N, 68 kDa TATA-binding protein-associated factor, TAF(II)68, TAFII68, RNA-binding protein 56

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, T-TB
clone   4D71, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  western blot: suitable
species reactivity   human
shipped in   wet ice
isotype   IgG1κ
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_003478
UniProt accession no.   Q92804
Gene Information   human ... TAF15(8148)

Description

General description

TAF15, also known as TATA-binding protein-associated factor 2N, or 68 kDa TATA-binding protein-associated factor, or TAF(II)68, TAFII68, or RNA-binding protein 56, and encoded by the gene TAF15/RBP56/TAF2N, is a RNA and ssDNA binding protein important in transcription initiation. TAF15 aligns with the RNA polymerase II (Pol II) multiprotein complex that together with other proteins like TBP initiates transcription. TAF15 is ubiquitously expressed, and TAF15 shuttles from the nucleus to the cytoplasm upon activation; methylation of TAF15 appears to guide nuclear localization and transcriptional activity. Translocations involving the TAF15 gene may play a role in acute leukemia and extraskeletal myxoid chondrosarcoma, and mutations in this gene are associated with amyotrophic lateral sclerosis (ALS) diseases.

Immunogen

Recombinant protein corresponding to human TAF15.

Application

Anti-TAF15 Antibody, clone 4D71 is an antibody against TAF15 for use in western blotting & ICC.

Immunocytochemistry Analysis: A 1:500 to a 1:1,000 dilution from a representative lot from an independent laboratory detected TAF15 in HeLa cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Target description

~68 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 μg/mL of this antibody detected TAF15 in 10 μg of HeLa cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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