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  • MABE949 - Anti-E3 Ubiquitin Ligase BRE1B Antibody, clone 4H8.1

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MABE949 Sigma-Aldrich

Anti-E3 Ubiquitin Ligase BRE1B Antibody, clone 4H8.1

Anti-E3 Ubiquitin Ligase BRE1B Antibody, clone 4H8.1 is an antibody against E3 Ubiquitin Ligase BRE1B for use in Western Blotting.

Synonym: E3 ubiquitin-protein ligase BRE1B, 95 kDa retinoblastoma-associated protein, BRE1-B, RBP95, RING finger protein 40, Syntaxin-1-interacting RING finger protein, Protein staring

  •  eCl@ss 32160702

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Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   100 µL (MABE949)
NCBI accession no.   NP_001193962
UniProt accession no.   O75150
biological source   mouse
application(s)   western blot: suitable
isotype   IgG1κ
clone   4H8.1, monoclonal
  monoclonal

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Target description

~120 kDa oberved. 113.7 kDa (isoform 1), 94.80 kDa (isoform 2), 102.0 kDa (isoform 3) calculated.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

General description

E3 ubiquitin-protein ligase BRE1B (UniProt O75150; also known as 95 kDa retinoblastoma-associated protein, BRE1-B, RBP95, RING finger protein 40, Syntaxin-1-interacting RING finger protein, Protein staring) is encoded by the RNF40 (also known as BRE1B, KIAA0661) gene (Gene ID 9810) in human. Proteini ubiquitination is mediated by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). There exist RING finger-type (really interesting new gene), HECT-type (homologous to E6-AP C-terminus), and U-box type of E3 ligases. RING finger ubiquitin ligase members can function as monomers, dimers, or multi-subunit complexes. The RNF20-RNF40 heterodimer mediates the ubiquitination of histone H2B at Lysine 120, an event that plays an important role in decompacting chromatin fiber to allow transcription elongation, in stem cell differentiation, as well as in DNA double-strand breaks (DSBs) repair via the homologous recombination (HR) and nonhomologous end-joining (NHEJ) pathways. Human RNF40 is a 1001-amino acid protein containing a RING-type zinc finger in its C-terminal region (a.a. 948-987) that mediates its interaction with RNF20.

Immunogen

Epitope: Internal (C-terminal half).

GST-tagged recombinant internal fragment from the C-terminal half of human BRE1B .

Other Notes

Concentration: Please refer to lot specific datasheet.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected BRE1B in 10 µg of HeLa cell lysate.

Specificity

Clone 4H8.1 epitope region is present in all three spliced isoforms of human BRE1B reported by UniProt (O75150).

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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