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MABF321 Sigma-Aldrich

Anti-CD45 (mouse) Antibody, PE, clone 30-F11

clone 30-F11, from rat, PE

Synonym: Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

conjugate   PE
clone   30-F11, monoclonal
biological source   rat
application(s)   activity assay: suitable
  flow cytometry: suitable
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
species reactivity   mouse
shipped in   wet ice
isotype   IgG2bκ
Quality Level   100
antibody product type   primary antibodies
UniProt accession no.   P06800
Gene Information   human ... PTPRC(5788)

Description

General description

CD45, also known as PTPRC, is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells. CD 45 belongs to the protein-tyrosine phosphatase family, receptor class 1/6 subfamily.It also contains 2 fibronectin type-III domains and 2 tyrosine-protein phosphatase domains. CD45 has been shown to play an important role in T-cell activation through the antigen receptor. CD45 also is suggested to act as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one is suggested to affect the substrate specificity of the first domain. Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 and FYN. Furthermore, it has been shown to dephosphorylate LYN, and modulate LYN activity. Interestingly, CD45 has been associated with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45. CD45 has been shown to bind with GANAB and PRKCSH. Additionally, it interacts with SKAP1 and DPP4, whereas the interaction is enhanced in a interleukin-12-dependent manner in activated lymphocytes. Mutations in CD45 have been associated with Multiple Sclerosis (MS) and severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels.

Application

Anti-CD45 (mouse) Antibody, PE, clone 30-F11 is a highly specific rat monoclonal antibody, that targets CD45 & has been tested in Flow Cytometry, IHC, Activity Assay, IP & western blotting.

Target description

145 kDa calculated

Quality

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.

Flow Cytometry Analysis: 0.25 μg of this antibody detected CD45 in one million C57BL/6 mouse splenocytes.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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