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MABF322 Sigma-Aldrich

Anti-CD45 (mouse) Antibody, PE/Cy5, clone 30-F11

Anti-CD45 (mouse) Antibody, PE/Cy5, clone 30-F11 is a highly specific rat monoclonal antibody, that targets CD45 & has been tested in Flow Cytometry, IHC, western blotting & IP.

Synonym: Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45

  •  eCl@ss 32160702

  •  NACRES NA.44

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Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   100 µg (MABF322)
application(s)   flow cytometry: suitable
  immunohistochemistry: suitable
  immunoprecipitation (IP): suitable
  western blot: suitable
biological source   rat
clone   30-F11, monoclonal
  monoclonal
isotype   IgG2bκ
shipped in   wet ice
species reactivity   mouse
gene symbol   PTPRC(5788)
  CD45
conjugate   PE-Cy5

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Target description

145 kDa calculated

Application

Research Category
Inflammation & Immunology

Research Sub Category
Cytokines & Cytokine Receptors

Western Blotting Analysis: A representative lot detected human CD45 in Western Blotting. (Tsuboi S and Fukuda M. 1998)

Immunohistochemistry Analysis: A representative lot detected human CD45 in Immunohistochemistry. (Simon DI, et al. 2000; Cornet A, et al. 2001)

Immunoprecipitation Analysis: A representative lot detected human CD45 in Immunoprecipitation. (Ledbetter JA, et al. 1979)

General description

CD45, also known as PTPRC, is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells. CD 45 belongs to the protein-tyrosine phosphatase family, receptor class 1/6 subfamily.It also contains 2 fibronectin type-III domains and 2 tyrosine-protein phosphatase domains. CD45 has been shown to play an important role in T-cell activation through the antigen receptor. CD45 also is suggested to act as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one is suggested to affect the substrate specificity of the first domain. Upon T-cell activation, CD45 recruits and dephosphorylates SKAP1 and FYN. Furthermore, it has been shown to dephosphorylate LYN, and modulate LYN activity. Interestingly, CD45 has been associated with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45. CD45 has been shown to bind with GANAB and PRKCSH. Additionally, it interacts with SKAP1 and DPP4, whereas the interaction is enhanced in a interleukin-12-dependent manner in activated lymphocytes. Mutations in CD45 have been associated with Multiple Sclerosis (MS) and severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels.

Immunogen

Mouse thymus or spleen

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

The antibody was purified by affinity chromatography, and conjugated with PE/Cy5 under optimal conditions. The solution is free of unconjugated PE/Cy5 and unconjugated antibody.

Flow Cytometry Analysis: 0.25 μg of this antibody detected CD45 in one million C57BL/6 mouse splenocytes.

Physical form

Affinity

Purified rat conjugate antibody in buffer containing PBS (pH 7.2) with up to 0.1% sodium azide.

Storage and Stability

The antibody solution should be stored undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Peer-Reviewed Papers
15

References

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