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MABF357 Sigma-Aldrich

Anti-CD45RO (human) Antibody, PE, clone UCHL1

Detect CD45RO using this mouse monoclonal antibody, Anti-CD45RO (human) Antibody, PE, clone UCHL1 validated for use in Flow Cytometry, western blotting, IHC & IP.

Synonym: Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45, CD45RO

  •  eCl@ss 32160702

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Properties

Related Categories Antibodies, Primary Antibodies More...
packaging   100 tests (MABF357)
gene symbol   PTPRC(5788)
  UCHL1(7345)
format   PE
molecular weight   147 kDa calculated
application(s)   Flow Cytometry
  Immunohistochemistry
  Immunoprecipitation
  Western Blotting
isotype   IgG2aκ
clone   Monoclonal Antibody
concentration   Please refer to the Certificate of Analysis for the lot-specific concentration.
host   Mouse
species reactivity   Human
gene symbol   CD45
UniProt accession no.   P08575
shipped in   1
Quality Assurance   The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.

Flow Cytometry Analysis: 5 μL of this antibody detected CD45RO in one million human peripheral blood lymphocytes.

Description

Application

Flow Cytometry Analysis: 5 μL from a representative lot detected CD45RO in one million human peripheral blood lymphocytes.

Western Blotting Analysis: A representative lot detected human CD45RO in Western Blotting. (Ishii T, et al. 2001.)

Immunohistochemistry Analysis: A representative lot detected human CD45RO in Immunohistochemistry. (Yamada M, et al.1996.;Sakkas LI, et al. 1998.)

Immunoprecipitation Analysis: A representative lot detected human CD45RO in Immunoprecipitation. (Ponsford M, et al. 2001.)





General description

Receptor-type tyrosine-protein phosphatase C, also known as Leukocyte common antigen (L-CA) or CD45 and encoded by the human gene PTPRC or CD45 is a transmembrane glycoprotein that belongs to the PTP family. PTPs are signaling molecules that regulate cellular process like cell growth, differentiation and mitotic cycle. CD45 has many isoforms that are distributed throughout the immune system according to cell type. These arise because of alternative splicing of RA, RB, and RC exons. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45RO is one of the isoforms of CD45 that facilitates T cell activation. It is also the shortest isform which lack the RA, RB, and RC exons. Mutations in CD45 have been associated with mulitple sclerosis and severe combined immunodeficiency.



Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Peer-Reviewed Papers
15

References

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