• USA Home
  • MABF41 - Anti-Interleukin-6 (IL-6) Antibody, clone 1G3

EMAIL THIS PAGE TO A FRIEND
MABF41 Sigma-Aldrich

Anti-Interleukin-6 (IL-6) Antibody, clone 1G3

clone AB42_51.38C8.1G3, from mouse

Synonym: interleukin 6 (interferon, beta 2), IFN-beta-2, B-cell stimulatory factor 2, interleukin-6, Interferon beta-2, B cell stimulatory factor-2, Hybridoma growth factor, B-cell differentiation factor, CTL differentiation factor, interleukin BSF-2

  •  eCl@ss 32160702

  •  NACRES NA.41

Purchase

Properties

Related Categories Antibodies, Antibodies for Cell Biology, Antibodies for Neurobiology, Antibodies to Chemokines, Antibodies to Cytokines and Growth Factors,
Quality Level   100
biological source   mouse
clone   AB42_51.38C8.1G3, monoclonal
species reactivity   human
application(s)   western blot: suitable
isotype   IgG2bκ
NCBI accession no.   NP_000591
UniProt accession no.   P05231
shipped in   wet ice

Description

General description

Interleukin-6 (IL-6) is a pro-inflammatory cytokine that seems to be involved in the late stages of CTL generation. Other biological functions also include amplification and the inflammatory response. Combination therapies including IL-6 have been shown to have positive effects on specific malignant tumor types. The accumulation of IL-6 in the CNS is usually detrimental and can contribute to pathology of CNS diseases. IL-6 signaling is negatively regulated by TGF-beta, which down regulates STAT1, STAT3 and ICAM-1 expression. IL-6 expression has been linked to inflammatory bowel disease (IBD) and is also the primary clinical target for IBD. Clinical use of an antibody for the IL-6 receptor (IL-6R) has been promising in treating rheumatoid arthritis, systemic juvenile idiopathic arthritis and Castleman’s disease.

Immunogen

Human IL-6 fused to the soluble domain of the IL-6R.

Application

Anti-Interleukin-6 (IL-6) Antibody, clone 1G3 is an antibody against Interleukin-6 (IL-6) for use in WB.

Research Category
Inflammation & Immunology

Research Sub Category
Cytokines & Cytokine Receptors

Quality

Evaluated by Western Blot in lysate from cells expressing human IL-6 recombinant protein.

Western Blot Analysis: 0.5 µg/mL of this antibody detected 10 µg of human IL-6 recombinant protein.

Target description

~21 kDa observed

Linkage

Replaces: 04-1095

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human IL-6 recombinant protein

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?