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  • MABF443 - Anti-CD45R (B220) Antibody (human/mouse), PE, clone RA3-6B2

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MABF443 Sigma-Aldrich

Anti-CD45R (B220) Antibody (human/mouse), PE, clone RA3-6B2

This rat monoclonal antibody Anti-CD45R (B220) (human/mouse), PE, clone RA3-6B2 validated for use in western blotting, IP, IHC, Immunofluorescence & Flow Cytometry for the detection of CD45R (B220).

Synonym: Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45

  •  eCl@ss 32160702

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Properties

Related Categories Antibodies, Primary Antibodies More...
gene symbol   Ly-5
  Ptprc
UniProt accession no.   P06800
Quality Assurance   The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.5 µg of this antibody detected CD45R (B220) in one million C57Bl/6 splenocytes.
application(s)   Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting, Immunocytochemistry
clone   Monoclonal Antibody
  RA3-6B2
concentration   0.2 mg/mL
format   PE
host   Rat
isotype   IgG2aκ
packaging   100 µg (MABF443)
shipped in   wet ice
species reactivity   Human, Mouse

Description

General description

The RA3-6B2 antibody reacts with the human and mouse CD45 isoform known as CD45R, or B220, a protein tyrosine phosphatase of 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns, all critical for leukocyte function. In mouse, the CD45R/B220 isoform is predominantly found on B cells, at varying levels on all stages from pro-B cells to activated B cells, and may also be detected on certain T cell and NK cell subsets. It is of note that B220 is not similarly expressed on human B cells, where it appears to be differentiation-specific and therefore expressed on only some B cell subsets. Other forms of CD45 with restricted cellular expression include CD45RA, CD45RB, CD45RO and several others.The RA3-6B2 antibody is one of the most consistently used leukocyte markers for B cells, T cell subsets and NK cell subsets in human and mouse. This antibody has also been reported as cross-reactive with feline CD45R/B220.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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